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Home Antibody All anti-HES1 antibodies

Anti-HES1 Antibody EPR4226

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307212
  • Rabbit monoclonal antibody against HES1(clone EPR4226)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC417251) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(1)
FC(1)
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Also for HES1 (NM_005524)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human HES1 was used as an immunogen.
Clone NameEPR4226 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; ICC: 1:100 - 1:250; FC: 1:100
Predicted MW Explanation 30 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens hes family bHLH transcription factor 1 (HES1)
Alternative NamebHLHb39; HES-1; HHL; HRY
Database LinkNP_005515
Entrez Gene 3280 Human
Entrez Gene 15205 Mouse
Entrez Gene 29577 Rat
FunctionHES1 belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. HES1 has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box (1).
Related PathwayStem cell relevant signaling - DSL/Notch pathwayES Cell Differentiation/IPSAdult stem cellsCancer stem cellsTranscription FactorsDruggable Genome Notch signaling pathwayMaturity onset diabetes of the young

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Hes1 antibody [EPR4226]; All lanes : Anti-Hes1 antibody [EPR4226] at 1/1000 dilution.Lane 1 : Fetal brain lysate.Lane 2 : PC12 cell lysate.Lane 3 : SH-SY5Y cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP-labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 30 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hes1 antibody [EPR4226]; TA307212 at 1/250 dilution staining Hes1 in paraffin-embedded Human placenta tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
FC Image
Flow Cytometry - Anti-Hes1 antibody [EPR4226]; Overlay histogram showing SH-SY5Y cells stained with TA307212 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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