OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-KRT16 antibodies

Anti-KRT16 Antibody EP1615Y

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307210
  • Rabbit monoclonal antibody against Cytokeratin 16 (clone EP1615Y )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC417219) , 20ug Explanation
100ul 325 3-7 Days
Add to Shopping Cart
WB(1)
IHC(1)
FC(1)
spacer
Also for KRT16 (NM_005557)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human CK-16 was used as an immunogen.
Clone NameEP1615Y IsotypeIgG
Species ReactivityHuman ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsIHC-P: 1:100 - 1:250; WB: 1:5000 - 1:10000; IP: 1:30; ICC: 1:100 - 1:250; FC: 1:50
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens keratin 16 (KRT16)
Alternative NameCK16; FNEPPK; K16; K1CP; KRT16A; NEPPK; PC1
Database LinkNP_005548
FunctionThe human type I keratin 16 (CK16) is constitutively expressed in a number of complex epithelial tissues, including skin, but is better known for its induction under conditions favoring enhanced proliferation or abnormal differentiation, including wound healing, psoriasis, and cancer (1). CK16 is expressed in suprabasal interfollicular epidermis in wound healing and other pathological conditions associated with hyperproliferation, such as psoriasis and are induced when keratinocytes are cultured in vitro. However, CK16 is also constitutively expressed in normal suprabasal mucosal and palmoplantar keratinocytes (2). Pachyonychia congenita (PC) is a group of inherited ectodermal dysplasias, the characteristic phenotype being hypertrophic nail dystrophy. The PC-1 phenotype may be distinguished by the absence of the epidermal cysts found in PC-2, and it has been shown to be caused by mutations in either keratin K16 or its expression partner, the K6a isoform of K6. Mutations in K16 have also been shown to cause a milder related phenotype, focal non-epidermolytic palmoplantar keratoderma (3).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Cytokeratin 16 antibody [EP1615Y]; Anti-Cytokeratin 16 [EP1615Y] antibody at 1/10000 dilution + HaCat cell lysate at 10 ug.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 51 kDa.Observed band size : 51 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 16 antibody [EP1615Y]; Immunohistochemical analysis of Cytokeratin 16 in paraffin embedded human squamous cervical carcinoma tissue using TA307210 at a 1/100 dilution.
FC Image
Flow Cytometry - Cytokeratin 16 antibody [EP1615Y]; Overlay histogram showing HepG2 cells stained with TA307210 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title