OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-NAMPT antibodies

Anti-NAMPT Antibody EPR3392

div

Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA307197 Rabbit monoclonal antibody against PBEF(clone EPR3392) 100ul $325 In Stock

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

spacer
WB(2)
IHC(7)
spacer
Also for NAMPT (NM_005746)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human PBEF was used as an immunogen.
Clone NameEPR3392 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:5000; IHC-P: 1:250 - 1:500; ICC: 1:50 - 1:100
Predicted MW Explanation 56 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens nicotinamide phosphoribosyltransferase (NAMPT)
Alternative Name1110035O14Rik; PBEF; PBEF1; VF; VISFATIN
Database LinkNP_005737
Entrez Gene 10135 Human
Entrez Gene 59027 Mouse
Entrez Gene 297508 Rat
FunctionPBEF (nicotinamide phosphoribosyltransferase) catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. This protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. PBEF is highly expressed in visceral fat, so serum levels of the protein correlate with obesity.
Related PathwayDruggable Genome Nicotinate and nicotinamide metabolism

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - PBEF antibody [EPR3392]; All lanes : Anti-Visfatin antibody [EPR3392] at 1/1000 dilution.Lane 1 : Fetal liver lysate.Lane 2 : Human skeletal muscle lysate.Lane 3 : Fetal kidney lysate.Lane 4 : Human heart lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 56 kDa.Observed band size : 52 kDa .
spacer
Western blot - Anti-PBEF antibody [EPR3392]; All lanes : Anti-Visfatin antibody [EPR3392] at 1/1000 dilution.Lane 1 : Mouse brain lysate.Lane 2 : Mouse heart lysate.Lane 3 : Mouse kidney lysate.Lane 4 : Mouse spleen lysate.Lane 5 : Rat brain lysate.Lane 6 : Rat heart lysate.Lane 7 : Rat kidney lysate.Lane 8 : Rat spleen lysate.Predicted band size : 56 kDa.Observed band size : 56 kDa.Additional bands at : 120 kDa,45 kDa. We are unsure as to the identity of these extra bands.Exposure time : 3 minutes
spacer
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PBEF antibody [EPR3392]; TA307197, at 1/250 dilution, staining PBEF in paraffin-embedded Human kidney tissue by Immunohistochemistry.
spacer
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PBEF antibody [EPR3392]; TA307197, at 1/250 dilution, staining PBEF in paraffin-embedded Human liver tissue by Immunohistochemistry.
spacer
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBEF antibody [EPR3392]; TA307197 staining at 1/250 dilution staining PBEF in mouse liver tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
spacer
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-PBEF antibody [EPR3392](TA307197); TA307197 showing positive staining in Normal placenta tissue.
spacer
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-PBEF antibody [EPR3392](TA307197); TA307197 showing negative staining in Normal tonsil tissue.
spacer
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-PBEF antibody [EPR3392](TA307197); TA307197 showing negative staining in Normal uterus tissue.
spacer
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-PBEF antibody [EPR3392](TA307197); TA307197 showing positive staining in Skeletal muscle tissue.
spacer

 

spacer
Inc 5000 Healthcare Company
All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title