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Anti-PSMD14 Antibody EPR4257


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SKU Description Amount Price Availability*  
  • Rabbit monoclonal antibody against POH1(clone EPR4257)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401764) , 20ug Explanation
100ul $325 3-7 Days
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Also for PSMD14 (NM_005805)
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human POH1 was used as an immunogen.
Clone NameEPR4257 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:500 - 1:1000; ICC/IF: 1:500 - 1:1000
Predicted MW Explanation 35 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 (PSMD14)
Alternative NamePAD1; POH1; RPN11
Database LinkNP_005796
Entrez Gene 10213 Human
Entrez Gene 59029 Mouse
Entrez Gene 311078 Rat
FunctionPOH1, also called PSMD14, is a member of the peptidase M67A family and a metalloprotease component of the 26S proteasome that specifically cleaves the 'Lys-63'-linked polyubiquitin chains. The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. It is widely expressed with highest levels in the heart and skeletal muscle (1).
Related PathwayProteaseDruggable Genome Proteasome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - PSMD14 antibody [EPR4257]; All lanes : Anti-PSMD14 antibody [EPR4257] at 1/1000 dilution.Lane 1 : HepG2 cell lysates.Lane 2 : A431 cell lysates.Lane 3 : HeLa cell lysates.Lane 4 : Namalwa cell lysates.Lane 5 : K562 cell lysates.Lane 6 : MCF7 cell lysates.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled Goat anti-Rabbit at 1/2000 dilution.Predicted band size : 35 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PSMD14 antibody [EPR4257]; TA307195, at 1/500 dilution staining PSMD14 in paraffin-embedded Human colon tissue, by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - PSMD14 antibody [EPR4257]; TA307195, at 1/500 dilution, staining PSMD14 in HeLa cells by Immunofluorescence.


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