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Home Antibody All anti-SMAD5 antibodies

Anti-SMAD5 PHOSPHO Antibody MMC-1-104-3

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307185
  • Rabbit monoclonal antibody against Smad5 Phospho (pS463/465) (clone MMC-11043 ) (Phospho-Specific)
100ul 379 3-7 Days
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WB(1)
IHC(8)

OriGene Data

ImmunogenA phospho-specific synthetic peptide corresponding to residues surrounding Serine 463 and 465 of human Smad5 was used as an immunogen.
Clone NameMMC-1-104-3 IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens SMAD family member 5 (SMAD5), transcript variant 1
Alternative NameDWFC; JV5-1; MADH5
Database LinkNP_005894
FunctionSmad5 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, eight Smads have been identified and can be divided into 3 subgroups based on their structure and function; pathway-restricted, common mediator and inhibitory. Smad 1, 5 and 8 serve as pathway-restricted Smads for the bone morphogenetic proteins (BMPs) signaling pathway (1). Smad5 is directly activated by BMP type Ia or Ib receptors upon BMP-2 stimulation and transduced BMP-2 signals to the nucleus by forming a complex with Smad 4, activating the transcription of the target gene in the nucleus (2-3). BMP activates Smad5 by phosphorylation at Ser463 and Ser465, which are the equivalent phosphorylation sites for Smad 1 and Smad 8.
Related Pathway
TGF Beta Signaling Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3]; All lanes : Anti-SMAD5 (phospho S463 + S465) [MMC-1-104-3] antibody at 1/500 dilution.Lane 1 : HeLa cell lysate (untreated).Lane 2 : HeLa cell lysate (treated with BMP-4).Lysates/proteins at 10 µg per lane.Secondary.HRP labelled Goat anti-Rabbit antibody at 1/2000 dilution.Predicted band size : 52 kDa.Observed band size : 58 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SMAD5 (phospho S463 + P465) antibody [MMC-1-104-3]; TA307185, at a 1/100 dilution, detecting SMAD5 (phospho S463 + S465) in paraffin embedded (1) Human breast carcinoma tissue and (2) Human colonic carcinoma tissue by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3](TA307185); TA307185 showing positive staining in Normal tonsil tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3](TA307185); TA307185 showing positive staining in Hepatocellular carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3](TA307185); TA307185 showing positive staining in Cervical carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3](TA307185); TA307185 showing positive staining in Glioma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3](TA307185); TA307185 showing positive staining in Hepatocellular carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3](TA307185); TA307185 showing positive staining in Cervical carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3](TA307185); TA307185 showing positive staining in Glioma tissue.

 

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