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Anti-MAPT PHOSPHO Antibody EPR2396(2)
Also for MAPT (NM_016841)
|A phospho specific peptide corresponding to residues surrounding threonine 50 of human Tau was used as an immunogen. This antibody only detects Tau phosphorylated at threonine 50.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:250 - 1:500; ICC/IF: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens microtubule-associated protein tau (MAPT), transcript variant 4|
|DDPAC; FTDP-17; MAPTL; MSTD; MTBT1; MTBT2; PPND; TAU|
|Tau is a microtubule binding protein that promotes microtubule assembly and stability. Tau is found to be the major component of the paired helical filaments (PHFs) found in the brains of patients with Alzheimer disease (AD) (1,2). Tau is hyperphosphorylated in PHFs, and specific phosphorylation sites have been implicated in the loss of Taus association with the membrane cortex during AD disease state, including Ser 199/202, Thr 231, and Ser 393/404 (3). Glycogen synthase kinase-3, or GSK-3, phosphorylates Tau on Ser 396 (4).|
MAPK signaling pathway
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Western blot - Tau (phospho T50) antibody [EPR2396(2)]; All lanes : Anti-Tau (phospho T50) antibody [EPR2396(2)] at 1/1000 dilution.Lane 1 : SH-SY5Y cell lysates, untreated.Lane 2 : SH-SY5Y cell lysates, treated with Okadiac acid and Calyculin A.Lysates/proteins at 10 µg per lane.Predicted band size : 79 kDa.
Tau (phospho T50) antibody [EPR2396(2)]; Immunohistochemical analysis of paraffin-embedded Human brain tissue using 1/250 ab109392.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Tau (phospho T50) antibody [EPR2396(2)]; Immunohistochemical analysis of paraffin-embedded Human glioma tissue using 1/250 ab109392.
Immunocytochemistry/ Immunofluorescence - Tau (phospho T50) antibody [EPR2396(2)]; Immunofluorescent staining of SHSY5Y cells using 1/100 ab109392.