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Home Antibody All anti-NUMA1 antibodies

Anti-NUMA1 Antibody EP3976

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307173
  • Rabbit monoclonal antibody against NuMA(clone EP3976)
100ul $325 3-7 Days
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WB(1)
IHC(3)
IF(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human NuMA was used as an immunogen.
Clone NameEP3976 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; ICC/IF: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant 1
Alternative NameNUMA
Database LinkNP_006176
FunctionNuMA (Nuclear mitotic apparatus protein 1) is an abundant 240 kD protein that is present in the nucleus of interphase cells and concentrates in the polar regions of the spindle apparatus during mitosis. Sequence analysis indicates that NuMA possesses an unusually long a-helical central region characteristic of many filament forming proteins. NuMA is resistant to high salt, detergent and nuclease extractions, indicating that it is a component of the nucleoskeleton (1). Overexpression of NuMA interferes with spindle-associated dynein localization and promotes multipolar spindle formation and cancer. On the other hand, NuMA is absent in many kinds of non-proliferating cells and highly differentiated cells. It also functions during meiotic spindle organization in male and female germ cells. Degradation of NuMA results in the breakdown of normal nuclear structure, and has been used as a marker of cell apoptosis (2).
Related Pathway
Cell cycle

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - NuMA antibody [EP3976]; All lanes : Anti-NuMA antibody [EP3976] at 1/1000 dilution.Lane 1 : K562 cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : MCF7 cell lysate.Lane 4 : Raji cell lysate.Lane 5 : SW480 cell lysate.Lane 6 : CACO2 cell lysate.Lane 7 : MOLT4 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 238 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NuMA antibody [EP3976]; TA307173, at 1/250 dilution, staining NuMA in paraffin-embedded Human breast carcinoma by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NuMA antibody [EP3976]; TA307173, at 1/250 dilution, staining NuMA in paraffin-embedded Human breast tissue by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NuMA antibody [EP3976]; TA307173, at 1/250 dilution, staining NuMA in paraffin-embedded Human colonic adenocarcinoma by Immunohistochemistry.
IF Image
Immunocytochemistry/ Immunofluorescence - NuMA antibody [EP3976]; TA307173, at 1/100 dilution, staining NuMA in HeLa cells by Immunofluorescence.

 

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