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Anti-NUMA1 Antibody EP3976
Also for NUMA1 (NM_006185)
|A synthetic peptide corresponding to residues in human NuMA was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens nuclear mitotic apparatus protein 1 (NUMA1), transcript variant 1|
Entrez Gene 4926 Human
Entrez Gene 101706 Mouse
Entrez Gene 308870 Rat
|NuMA (Nuclear mitotic apparatus protein 1) is an abundant 240 kD protein that is present in the nucleus of interphase cells and concentrates in the polar regions of the spindle apparatus during mitosis. Sequence analysis indicates that NuMA possesses an unusually long a-helical central region characteristic of many filament forming proteins. NuMA is resistant to high salt, detergent and nuclease extractions, indicating that it is a component of the nucleoskeleton (1). Overexpression of NuMA interferes with spindle-associated dynein localization and promotes multipolar spindle formation and cancer. On the other hand, NuMA is absent in many kinds of non-proliferating cells and highly differentiated cells. It also functions during meiotic spindle organization in male and female germ cells. Degradation of NuMA results in the breakdown of normal nuclear structure, and has been used as a marker of cell apoptosis (2).|
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Western blot - NuMA antibody [EP3976]; All lanes : Anti-NuMA antibody [EP3976] at 1/1000 dilution.Lane 1 : K562 cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : MCF7 cell lysate.Lane 4 : Raji cell lysate.Lane 5 : SW480 cell lysate.Lane 6 : CACO2 cell lysate.Lane 7 : MOLT4 cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 238 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NuMA antibody [EP3976]; TA307173, at 1/250 dilution, staining NuMA in paraffin-embedded Human breast carcinoma by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NuMA antibody [EP3976]; TA307173, at 1/250 dilution, staining NuMA in paraffin-embedded Human breast tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NuMA antibody [EP3976]; TA307173, at 1/250 dilution, staining NuMA in paraffin-embedded Human colonic adenocarcinoma by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - NuMA antibody [EP3976]; TA307173, at 1/100 dilution, staining NuMA in HeLa cells by Immunofluorescence.