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Anti-TNFAIP3 Antibody EPR2663
Also for TNFAIP3 (NM_006290)
|A synthetic peptide corresponding to residues in human TNFAIP3 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; ICC: 1:100; FC: 1:50
|Does not react with Mouse, Rat. Is unsuitable for IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens tumor necrosis factor, alpha-induced protein 3 (TNFAIP3), transcript variant 3|
|A20; OTUD7C; TNFA1P2|
|Tumor necrosis factor, alpha-induced protein 3 (TNFAIP3) is a de-ubiquitinating enzyme that contains an N-terminal catalytic domain belonging to the ovarian-tumour superfamily of cysteine proteases (1). TNFAIP3 suppresses apoptosis. It is expressed in various cell types in response to a variety of stimuli that activate the transcription factor NF-kB, including Il-1, CD40 ligand, and PMA (3). TNFAIP3 has been strongly suggested as a key protein involved in tamoxifen resistance, and thus represents both a new breast cancer marker and a promising target for developing new strategies to prevent the emergence of acquired mechanisms of drug resistance in breast cancer (2). |
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Western blot - TNFAIP3 antibody [EPR2663]; All lanes : Anti-TNFAIP3 antibody [EPR2663] at 1/1000 dilution.Lane 1 : Jurkat cells treated with TNF and TPA.Lane 2 : Daudi cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit IgG at 1/2000 dilution.Predicted band size : 90 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TNFAIP3 antibody [EPR2663]; ab92324 at 1/50 dilution, staining TNFAIP3 in paraffin embedded Human testis tissue
Immunocytochemistry/ Immunofluorescence - Anti-TNFAIP3 antibody [EPR2663]; ICC/IF image of ab92324 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Flow Cytometry - Anti-TNFAIP3 antibody [EPR2663]; Overlay histogram showing HepG2 cells stained with ab92324 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.