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Home Antibody All anti-TNFAIP3 antibodies

Anti-TNFAIP3 Antibody EPR2663

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307167
  • Rabbit monoclonal antibody against TNFAIP3 (clone EPR2663 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC416749) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for TNFAIP3 (NM_006290)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human TNFAIP3 was used as an immunogen.
Clone NameEPR2663 IsotypeTissue culture supernatant
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; ICC: 1:100; FC: 1:50
BufferDoes not react with Mouse, Rat. Is unsuitable for IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens tumor necrosis factor, alpha-induced protein 3 (TNFAIP3), transcript variant 3
Alternative NameA20; OTUD7C; TNFA1P2
Database LinkNP_006281
Entrez Gene 7128 Human
FunctionTumor necrosis factor, alpha-induced protein 3 (TNFAIP3) is a de-ubiquitinating enzyme that contains an N-terminal catalytic domain belonging to the ovarian-tumour superfamily of cysteine proteases (1). TNFAIP3 suppresses apoptosis. It is expressed in various cell types in response to a variety of stimuli that activate the transcription factor NF-kB, including Il-1, CD40 ligand, and PMA (3). TNFAIP3 has been strongly suggested as a key protein involved in tamoxifen resistance, and thus represents both a new breast cancer marker and a promising target for developing new strategies to prevent the emergence of acquired mechanisms of drug resistance in breast cancer (2).
Related PathwayDruggable Genome NOD-like receptor signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - TNFAIP3 antibody [EPR2663]; All lanes : Anti-TNFAIP3 antibody [EPR2663] at 1/1000 dilution.Lane 1 : Jurkat cells treated with TNF and TPA.Lane 2 : Daudi cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit IgG at 1/2000 dilution.Predicted band size : 90 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TNFAIP3 antibody [EPR2663]; TA307167 at 1/50 dilution, staining TNFAIP3 in paraffin embedded Human testis tissue
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-TNFAIP3 antibody [EPR2663]; ICC/IF image of TA307167 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was DyLight488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
FC Image
Flow Cytometry - Anti-TNFAIP3 antibody [EPR2663]; Overlay histogram showing HepG2 cells stained with TA307167 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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