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Anti-MCAM Antibody EPR3207(2)
Also for MCAM (NM_006500)
|A synthetic peptide corresponding to residues in human CD146 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; ICC/IF: 1:250 - 1:500
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,IHC-P or IP.|
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,IHC-P or IP.
|Homo sapiens melanoma cell adhesion molecule (MCAM)|
|CD146 (melanoma-associated antigen A32 or MUC18) is a transmembrane glycoprotein expressed on endothelial cells and is located at the intercellular junction where it plays a role in cell adhesion, and in the cohesion of the endothelial monolayer. CD146 engagement initiates an outside-in signaling pathway involving the protein tyrosine kinases FYN and FAK as well as Paxillin. In addition, it is a signaling molecule involved in the dynamics of actin cytoskeleton rearrangement (1). CD146 is also found to be associated with tumor progression and the development of metastasis in human malignant melanoma. Expression of CD146 is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage (2).|
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Western blot - Anti-CD146 antibody [EPR3207(2)]; All lanes : Anti-CD146 antibody [EPR3207(2)] at 1/1000 dilution.Lane 1 : A375 cell lysate.Lane 2 : Fetal artery lysate.Lane 3 : HUVEC cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 72 kDa.
Immunocytochemistry/ Immunofluorescence - Anti-CD146 antibody [EPR3207(2)]; Immunofluorescent analysis of HeLa cells labelling CD146 with ab134065 at 1/250 dilution.