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Home Antibody All anti-KHDRBS1 antibodies

Anti-KHDRBS1 Antibody EPR3232

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SKU Description Amount Price Availability*  
TA307153
  • Rabbit monoclonal antibody against SAM68(clone EPR3232)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC416574) , 20ug Explanation
100ul $325 3-7 Days
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for KHDRBS1 (NM_006559)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human SAM68 was used as an immunogen.
Clone NameEPR3232 IsotypeIgG
Species ReactivityMouse, Human (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:250 - 1:500; FC: 1:100 - 1:1000; ICC/IF: 1:250 - 1:500
Predicted MW Explanation 48 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Rat

Reference Data

Target NameHomo sapiens KH domain containing, RNA binding, signal transduction associated 1 (KHDRBS1), transcript variant 1
Alternative Namep62; p68; Sam68
Database LinkNP_006550
Entrez Gene 10657 Human
Entrez Gene 20218 Mouse
FunctionSAM68 (Src-associated protein in mitosis 68 kDa) is a member of the signal transduction and activation of RNA family of KH domain-containing RNA binding proteins (1). It is a major substrate of the Src tyrosine kinase in mitotic cells, having a tyrosine-rich C-terminal region and six polyproline (SH3-binding) sites, many of which are located in an amino-terminal region. Once phosphorylated, SAM68 functions as an adaptor protein in signal transduction cascades by binding to SH2- and SH3-domain containing proteins (2). Tyrosine phosphorylation also negatively regulates the nucleic acid binding properties of SAM68 (3).
Related PathwayTranscription Factors

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - SAM68 antibody [EPR3232]; All lanes : Anti-SAM68 antibody [EPR3232] at 1/1000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : A431 cell lysate.Lane 4 : A673 cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 48 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SAM68 antibody [EPR3232]; TA307153, at 1/250, staining SAM68 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
IF Image
Immunocytochemistry/ Immunofluorescence - SAM68 antibody [EPR3232]; TA307153, at 1/250, staining SAM68 in HeLa cells by Immunofluorescence.
FC Image
Flow Cytometry - Anti-SAM68 antibody [EPR3232]; Overlay histogram showing HeLa cells stained with TA307153 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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