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Home Antibody All anti-KHDRBS1 antibodies

Anti-KHDRBS1 Antibody EPR3232

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307153
  • Rabbit monoclonal antibody against SAM68(clone EPR3232)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC416574) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for KHDRBS1 (NM_006559)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human SAM68 was used as an immunogen.
Clone NameEPR3232 IsotypeIgG
Species ReactivityMouse, Human (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:250 - 1:500; FC: 1:100 - 1:1000; ICC/IF: 1:250 - 1:500
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Rat

Reference Data

Target NameHomo sapiens KH domain containing, RNA binding, signal transduction associated 1 (KHDRBS1), transcript variant 1
Alternative Namep62; p68; Sam68
Database LinkNP_006550
Entrez Gene 10657 Human
Entrez Gene 20218 Mouse
FunctionSAM68 (Src-associated protein in mitosis 68 kDa) is a member of the signal transduction and activation of RNA family of KH domain-containing RNA binding proteins (1). It is a major substrate of the Src tyrosine kinase in mitotic cells, having a tyrosine-rich C-terminal region and six polyproline (SH3-binding) sites, many of which are located in an amino-terminal region. Once phosphorylated, SAM68 functions as an adaptor protein in signal transduction cascades by binding to SH2- and SH3-domain containing proteins (2). Tyrosine phosphorylation also negatively regulates the nucleic acid binding properties of SAM68 (3).
Related PathwayTranscription Factors

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - SAM68 antibody [EPR3232]; All lanes : Anti-SAM68 antibody [EPR3232] at 1/1000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : A431 cell lysate.Lane 4 : A673 cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 48 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SAM68 antibody [EPR3232]; TA307153, at 1/250, staining SAM68 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
IF Image
Immunocytochemistry/ Immunofluorescence - SAM68 antibody [EPR3232]; TA307153, at 1/250, staining SAM68 in HeLa cells by Immunofluorescence.
FC Image
Flow Cytometry - Anti-SAM68 antibody [EPR3232]; Overlay histogram showing HeLa cells stained with TA307153 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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