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Anti-YWHAE Antibody EPR3918
Also for YWHAE (NM_006761)
|A synthetic peptide corresponding to residues in human 14-3-3E was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IF, FC
||ICC/IF: 1:100 - 1:250; WB: 1:1000 - 1:10000; FC: 1:10 - 1:1000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for IHC-P or IP.
|Homo sapiens tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon (YWHAE), transcript variant 1|
|14-3-3E; HEL2; KCIP-1; MDCR; MDS|
|The 14-3-3 epsilon protein (14-3-3E) is one of seven isoforms in the 14-3-3 protein family; a family which consists of acidic 30 kDa proteins expressed at high levels in neurons of the central nervous system (1). It is an adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. 14-3-3E binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif (2). It also interacts with both, the insulin-like growth factor I receptor (IGFIR) and the insulin receptor substrate I (IRS-1), suggesting its role in the regulation of insulin sensitivity (3). 14-3-3E has also been implicated in the pathogenesis of small cell lung cancer (4). |
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Western blot - 14-3-3 epsilon antibody [EPR3918]; All lanes : Anti-14-3-3 epsilon antibody [EPR3918] at 1/1000 dilution.Lane 1 : Human fetal brain cell lysate.Lane 2 : SH-SY5Y cell lysate.Lane 3 : 293T cell lysate.Lane 4 : SW480 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 29 kDa.
Immunocytochemistry/ Immunofluorescence - 14-3-3 epsilon antibody [EPR3918]; TA307143 at 1/100 dilution staining 14-3-3 epsilon in HeLa cells by Immunofluorescence.
Flow Cytometry - Anti-14-3-3 epsilon antibody [EPR3918]; Overlay histogram showing SH-SY5Y cells stained with TA307143 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.