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Anti-PARK7 Antibody EP2816Y
Also for PARK7 (NM_007262)
|A synthetic peptide corresponding to residues near the internal region of human DJ-1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||FC: 1:50, ICC: 1:50 - 100, IHC: 1:100 - 250, WB: 1:10,000 - 20,000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens parkinson protein 7 (PARK7), transcript variant 1|
|DJ-1 is ubiquitously expressed in various human tissues, and expression is induced by growth stimuli. Moreover, DJ-1 translocates from cytoplasm to nuclei in the S phase of the cell cycle. DJ-1 is thus suggested to be a novel mitogen-dependent oncogene product involved in a Ras-related signal transduction pathway (1). DJ-1 was first identified as a novel candidate of the oncogene product that transformed mouse NIH3T3 cells in cooperation with an activated ras. Later DJ-1 was also found to be an infertility-related protein that was reduced in rat sperm treated with sperm toxicants that cause infertility in rats. Results of further tests indicate that DJ-1 is a positive regulator of the androgen receptor (2). Mutations in a gene on chromosome 1, DJ-1, have been reported recently to be associated with recessive, earlyonset Parkinson's disease. The L166P mutation has the simple effect of promoting DJ-1 degradation, thereby reducing net DJ-1 protein within the cell (3). |
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Western blot - DJ1 antibody [EP2816Y]; All lanes : Anti-PARK7/DJ1 antibody [EP2816Y] at 1/20000 dilution.Lane 1 : TF-1 cell lysate.Lane 2 : Jurkat cell lysate.Lane 3 : HeLa cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/1000 dilution.Predicted band size : 20 kDa.Observed band size : 24 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - DJ1 antibody [EP2816Y]; Immunohistochemical analysis of paraffin-embedded human brain tissue using TA307131 at 1/100 dilution.
Flow Cytometry - Anti-PARK7/DJ1 antibody [EP2816Y]; Overlay histogram showing Jurkat cells stained with TA307131 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22Â°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22Â°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1Âµg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.