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Anti-MME Antibody EPR2997
|A synthetic peptide corresponding to residues on the N-terminus of human CD10 was used as an immogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:5000 - 1:10000; FC: 1:100 - 1:150
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens membrane metallo-endopeptidase (MME), transcript variant 1bis|
|CALLA; CD10; NEP; SFE|
|CD10 is zinc-dependant metalloprotease enzyme that plays a role in the destruction of opiod peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (1). CD10 is a cell-surface glycoprotein expressed on most acute lymphoblastic leukemias and certain other immature lymphoid malignancies and on normal lymphoid progenitors. The latter are either uncommitted to B- or T-cell lineage or committed to only the earliest stages of B- or T-lymphocyte maturation. CD10 is not restricted to leukemic cells, however, and is found on a variety of normal tissues. CD10 is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium (2). The proteolysis of beta-amyloid (Abeta) requires CD10, an enzyme that has been shown as reduced in Alzheimer's disease (AD). The defect of CD10 appears to correlate with Abeta deposition but not with degeneration and dementia (3). |
Hematopoietic cell lineage
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Western blot - CD10 antibody [EPR2997]; All lanes : Anti-CD10 antibody [EPR2997] at 1/10000 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : LnCap cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 86 kDa.Observed band size : 100 kDa .
Flow Cytometry-CD10 antibody [EPR2997](ab79423); Overlay histogram showing Jurkat cells stained with ab79423 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.