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Anti-Parva PHOSPHO Antibody EPR2174
Also for Parva (NM_018222)
|A phospho specific peptide corresponding to residues surrounding serine 8 of human Parvin was used as an immunogen. This antibody detects Actopaxin phosphorlyated on serine 8.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||ICC/IF: 1:100 - 1:250; WB: 1:2500 - 1:5000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,IHC-P or IP.
|Homo sapiens parvin, alpha (PARVA)|
|Actopaxin (also named Parvin, Parva) is a member of the alpha actinin superfamily containing a single alpha-actinin-like actin-binding domain. The Parvin family plays a role in cell adhesion and cytoskeleton organization. Analysis of Actopaxin and its para- and orthologues suggest, that the parvins represent a new family of alpha-actinin-related proteins that mediate cell-matrix adhesion (1). Actopaxin as a widely expressed protein that binds directly to both F-actin and paxillin LD1 and LD4 motifs. It exhibits robust focal adhesion localization in several cultured cell types but is not found along the length of the associated actin-rich stress fibers. Actopaxin colocalizes with paxillin to rudimentary focal complexes at the leading edge of migrating cells. Results suggest an important role for actopaxin in integrin-dependent remodeling of the actin cytoskeleton during cell motility and cell adhesion (2). |
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Western blot - Parvin alpha (phospho S8) antibody [EPR2174]; All lanes : Anti-Parvin alpha (phospho S8) antibody [EPR2174] at 1/5000 dilution.Lane 1 : A549 cell lysate.Lane 2 : A549 cell lysate treated with LP.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 42 kDa.Observed band size : 42 kDa.
Immunocytochemistry/ Immunofluorescence - Parvin alpha (phospho S8) antibody [EPR2174]; Immunofluorescent staining of HeLa cells using ab79409 at 1/100 dilution.