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Anti-XRCC5 Antibody EPR3468
Also for XRCC5 (NM_021141)
|A synthetic peptide corresponding to residues in human Ku80 was used as an immunogen.|
||Tissue culture supernatant
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:1000 - 1:10000; IP: 1:50; IHC-P: 1:250 - 1:500; ICC/IF: 1:500 - 1:1000
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for Flow Cyt.
|Homo sapiens X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) (XRCC5)|
|KARP-1; KARP1; KU80; Ku86; KUB2; NFIV|
|The Ku antigen is a DNA-associated nuclear protein originally recognized by the sera of patients with autoimmune diseases (1). It plays a key role in multiple nuclear processes, such as DNA repair, chromosome maintenance, transcription regulation, and V(D)J recombination (2). The Ku protein is composed of 70 and 82 kDa subunits (Ku70 and Ku80, respectively) and contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining pathway (3). When bound to DNA, the Ku 70/80 heterodimer enhances the kinase activity of the catalytic subunit of the DNA-dependent protein kinase, DNA-PKcs (4). |
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Western blot - Ku80 antibody [EPR3468]; All lanes : Anti-Ku80 antibody [EPR3468] at 1/50000 dilution.Lane 1 : A549 cell lysate .Lane 2 : HeLa cell lysate.Lane 3 : HepG2 cell lysate.Lane 4 : MCF7 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 83 kDa.Observed band size : 83 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ku80 antibody [EPR3468]; Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using 1/100 TA307065.
Immunocytochemistry/ Immunofluorescence - Ku80 antibody [EPR3468]; Immunofluorescence analysis of HeLa cells with 1/500 TA307065.