OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-RELA antibodies

Anti-RELA PHOSPHO Antibody EPR2379

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307058
  • Rabbit monoclonal antibody against NF-kappa-B p65 Phospho (pS529)(clone EPR2379) (Phospho-Specific)
100ul $325 3-7 Days
Add to Shopping Cart
WB(1)
spacer
Also for RELA (NM_021975)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding serine 529 of human NF-kappa-B was used as an immunogen. This antibody only detects NF-kappa-B phosphorylated at serine 529.
Clone NameEPR2379 IsotypeTissue culture supernatant
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; FC: 1:100 - 1:500
BufferDoes not react with Mouse, Rat. Is unsuitable for ICC or IHC-P.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for ICC or IHC-P.

Reference Data

Target NameHomo sapiens v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA), transcript variant 1
Alternative NameNFKB3; p65
Database LinkNP_068810
FunctionNuclear factor B (NF-B) encompasses an important family of inducible transcriptional activators that regulate a wide variety of cellular and viral genes (1). They play a role as transcription factors in the adaptive immune response, inflammation, and apoptosis (2). The p65 subunit, similar to two others in the B family, RelB and c-Rel, contains two transactivation domains in the C-terminal region of the protein (3). Association with inhibitory proteins of the IκB family retains NF-κB in the cytoplasm. Degradation of IB proteins exposes the nuclear localization sequence (NLS), leading to nuclear translocation and subsequent binding of NF-κB to DNA (4). p65 phosphorylation events occur in the cytoplasm or nucleus and are stimuli- and cell-type specific, on serines 276, 529, and 536 by various kinases: i.e. PKA, casein kinase II, IKK beta, and RSK1. Phosphorylation on these sites has been linked to the regulation of NF-κB activation (5).
Related Pathway
Apoptosis
Delta-Notch Signaling Pathway
MAPK signaling pathway
Toll-like receptor signaling pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - NFkB p65 (phospho S529) antibody [EPR2379]; All lanes : Anti-NF-kB p65 (phospho S529) antibody [EPR2379] at 1/1000 dilution.Lane 1 : Daudi cell lysates untreated with Calyculin A and TNF-alpha.Lane 2 : Daudi cell lysates treated with Calyculin A and TNF-alpha.Lysates/proteins at 10 µg per lane.Secondary.HRP-labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 60 kDa.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title