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Anti-RELA PHOSPHO Antibody EPR2379
Also for RELA (NM_021975)
|A phospho specific peptide corresponding to residues surrounding serine 529 of human NF-kappa-B was used as an immunogen. This antibody only detects NF-kappa-B phosphorylated at serine 529.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; FC: 1:100 - 1:500
|Does not react with Mouse, Rat. Is unsuitable for ICC or IHC-P.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for ICC or IHC-P.
|Homo sapiens v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA), transcript variant 1|
|Nuclear factor B (NF-B) encompasses an important family of inducible transcriptional activators that regulate a wide variety of cellular and viral genes (1). They play a role as transcription factors in the adaptive immune response, inflammation, and apoptosis (2). The p65 subunit, similar to two others in the B family, RelB and c-Rel, contains two transactivation domains in the C-terminal region of the protein (3). Association with inhibitory proteins of the IκB family retains NF-κB in the cytoplasm. Degradation of IB proteins exposes the nuclear localization sequence (NLS), leading to nuclear translocation and subsequent binding of NF-κB to DNA (4). p65 phosphorylation events occur in the cytoplasm or nucleus and are stimuli- and cell-type specific, on serines 276, 529, and 536 by various kinases: i.e. PKA, casein kinase II, IKK beta, and RSK1. Phosphorylation on these sites has been linked to the regulation of NF-κB activation (5).|
Delta-Notch Signaling Pathway
MAPK signaling pathway
Toll-like receptor signaling pathway
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Western blot - NFkB p65 (phospho S529) antibody [EPR2379]; All lanes : Anti-NF-kB p65 (phospho S529) antibody [EPR2379] at 1/1000 dilution.Lane 1 : Daudi cell lysates untreated with Calyculin A and TNF-alpha.Lane 2 : Daudi cell lysates treated with Calyculin A and TNF-alpha.Lysates/proteins at 10 µg per lane.Secondary.HRP-labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 60 kDa.