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Home Antibody All anti-NANOG antibodies

Anti-NANOG Antibody EPR2027(2)

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307042
  • Rabbit monoclonal antibody against Nanog(clone EPR2027(2))
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC411030) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(5)
IF(2)
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Also for NANOG (NM_024865)
cDNA Clone shRNA/siRNA Primer Pair Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human Nanog was used as an immunogen.
Clone NameEPR2027(2) IsotypeTissue culture supernatant
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt or IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens Nanog homeobox (NANOG), transcript variant 1
Alternative Namehomeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; Nanog homeobox
Database LinkNP_079141
Entrez Gene 79923 Human
FunctionNanog is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Nanog has been reported to block bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (1).
Related PathwayES Cell Differentiation/IPSStem cell - PluripotencyCancer stem cellsEmbryonic stem cellsInduced pluripotent stem cells

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Nanog antibody [EPR2027(2)]; Anti-Nanog antibody [EPR2027(2)] at 1/1000 dilution + NCCIT cell lysate at 10 ug.Predicted band size : 35 kDa.Observed band size : 37 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nanog antibody [EPR2027(2)]; TA307042, at a 1/100 dilution, staining Nanog in paraffin embedded Human seminoma tissue by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nanog antibody [EPR2027(2)]; TA307042, at a 1/100 dilution, staining Nanog in paraffin embedded Human dysgerminoma tissue by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)]; TA307042 showing negative staining in Fetal colon tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)]; TA307042 showing positive staining in Embryonal carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody [EPR2027(2)]; TA307042 showing negative staining in Normal colon tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Nanog antibody [EPR2027(2)]; TA307042, at a 1/100 dilution, staining Nanog in embryonic carcinoma cell line by Immunofluorescence.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [EPR2027(2)]; TA307042 staining NANOG in Human Liver cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 1% BSA for 12 hours at 4°C. Sample was incubated with primary antibody (1/500 in PBS) for 16 hour at 4°C. An Alexa Fluor?647-conjugated Donkey anti-rabbit(1/1000) IgG polyclonal was used as the secondary antibody

 

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