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Anti-ATG4A Antibody EPR4122
Also for ATG4A (NM_052936)
|A synthetic peptide corresponding to residues in human ATG4A was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or ICC.
|Homo sapiens autophagy related 4A, cysteine peptidase (ATG4A), transcript variant 1|
|Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging (1). ATG4A is a cysteine protease required for autophagy, which cleaves the C-terminal part of target proteins, such as MAP1LC3, GABARAPL2 and GABARAP. ATG4A belongs to the peptidase C54 family, and is widely with high expression level in skeletal muscle and brain (2).|
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Western blot - ATG4A antibody [EPR4122]; All lanes : Anti-ATG4A antibody [EPR4122] at 1/1000 dilution.Lane 1 : Fetal liver lysate.Lane 2 : HepG2 lysate.Lane 3 : Jurkat lysate.Lane 4 : Fetal brain lysate.Lysates/proteins at 10 µg per lane.Secondary.Standard HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 45 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ATG4A antibody [EPR4122]; Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab108322 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ATG4A antibody [EPR4122]; Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue using ab108322 at 1/100 dilution.