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Anti-STAT1 PHOSPHO Antibody EPR3146


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SKU Description Amount Price Availability*  
TA307009 Rabbit monoclonal antibody against Stat-1 Phospho (pS727)(clone EPR3146) (Phospho-Specific) 100ul $325 In Stock
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OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding Serine 727 of human Stat-1 was used as an immunogen. This antibody only detects Stat-1 phosphorylated at Serine 727.
Clone NameEPR3146 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC: 1:250 - 1:500
Predicted MW Explanation 87 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IP.

Reference Data

Target NameHomo sapiens signal transducer and activator of transcription 1 (STAT1), transcript variant alpha
Alternative NameCANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
Database LinkNP_009330
Entrez Gene 6772 Human
Entrez Gene 20846 Mouse
Entrez Gene 25124 Rat
FunctionStat-1 is a latent transcription factor that functions both as a signal transducer in the cytoplasm and an activator of transcription in the nucleus. Stat-1 is one of seven members of the Signal transducer and activators of transcription (STAT) family (1). Stat-1 is activated by a large number of ligands: IFN-alpha, IFN-gamma, EGF, PDGF and IL6 (2-3). IFN-gamma signaling induces phosphorylation of the Stat-1 residue Tyrosine 701, which facilitates dimerization, nuclear translocation, and DNA binding (4). Upon tyrosine phosphorylation, Stat-1 dimerizes with Stat-2 and associates with ISGF3/IR9 to form a complex called the ISGF3 transcription factor (5). This activation subsequently allows the complex to translocate to the nucleus and bind specific enhancer elements. Additional complexes can form upon IFN stimulation (1). Defects in Stat-1 are the cause of Stat-1 deficiency, characterized by mycobacterial or viral diseases (6).
Related PathwayTranscription FactorsDruggable Genome Chemokine signaling pathwayToll-like receptor signaling pathwayJak-STAT signaling pathwayPathways in cancerPancreatic cancer

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - STAT1 (phospho S727) antibody [EPR3146]; All lanes : Anti-STAT1 (phospho S727) antibody [EPR3146] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : HeLa cell lysate treated with Alkaline Phosphatase (AP).Lysates/proteins at 10 ug per lane.Secondary.HRP-labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 87 kDa.Observed band size : 91 kDa .

IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - STAT1 (phospho S727) antibody [EPR3146]; TA307009, at a 1/100 dilution, staining STAT1 (phospho S727) in paraffin embedded Human stomach adenocarcinoma tissue by Immunohistochemistry.


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