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Anti-STAT1 PHOSPHO Antibody EPR3147
Also for STAT1 (NM_007315)
|A phospho specific peptide corresponding to residues surrounding Tyrosine 701 of human Stat-1 was used as an immunogen. This antibody only detects Stat-1 phosphorylated at Tyrosine 701.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
|Homo sapiens signal transducer and activator of transcription 1, 91kDa (STAT1), transcript variant alpha|
|CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91|
Entrez Gene 6772 Human
|Stat-1 is a latent transcription factor that functions both as a signal transducer in the cytoplasm and an activator of transcription in the nucleus. Stat-1 is one of seven members of the Signal transducer and activators of transcription (STAT) family (1). Stat-1 is activated by a large number of ligands: IFN-alpha, IFN-gamma, EGF, PDGF and IL6 (2-3). IFN-gamma signaling induces phosphorylation of the Stat-1 residue Tyrosine 701, which facilitates dimerization, nuclear translocation, and DNA binding (4). Upon tyrosine phosphorylation, Stat-1 dimerizes with Stat-2 and associates with ISGF3/IR9 to form a complex called the ISGF3 transcription factor (5). This activation subsequently allows the complex to translocate to the nucleus and bind specific enhancer elements. Additional complexes can form upon IFN stimulation (1). Defects in Stat-1 are the cause of Stat-1 deficiency, characterized by mycobacterial or viral diseases (6).|
|Transcription FactorsDruggable Genome Chemokine signaling pathwayToll-like receptor signaling pathwayJak-STAT signaling pathwayPathways in cancerPancreatic cancer|
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Western blot - STAT1 alpha (phospho Y701) antibody [EPR3147]; All lanes : Anti-STAT1 alpha (phospho Y701) antibody [EPR3147] at 1/1000 dilution.Lane 1 : A431 cell lysates untreated with Epidermal Growth Factor (EGF).Lane 2 : A431 cell lysates treated with Epidermal Growth Factor (EGF).Lysates/proteins at 10 ug per lane.Secondary.HRP-labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 87 kDa.