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Home Antibody All anti-RAB27A antibodies

Anti-RAB27A Antibody EPR3021

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA306983
  • Rabbit monoclonal antibody against RAB27A(clone EPR3021)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC405229) , 20ug
100ul $325 3-7 Days
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WB(1)
IHC(1)
IF(1)
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Also for RAB27A (NM_183234)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human RAB27A was used as an immunogen.
Clone NameEPR3021 IsotypeIgG
Species ReactivityHuman, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:100 - 1:500; ICC/IF: Use at an assay dependent concentration
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse

Reference Data

Target NameHomo sapiens RAB27A, member RAS oncogene family (RAB27A), transcript variant 2
Alternative NameGS2; HsT18676; RAB27; RAM
Database LinkNP_899057
FunctionRas-related protein (RAB27A), a member of the Ras family, is a GTP-binding protein that is involved in protein transport and small GTPase mediated signal transduction (1). RAB27A plays a role in cytotoxic granule exocytosis in lymphocytes (1). This process is mediated by RAB27A acting as an effector for JFC1/Slp1 and Munc13-4 (2). RAB27A also interacts with Slp4-a (synaptotagmin-like protein4-a) to negatively control dense-core vesicle exocytosis (3). In addition, it is required for granule maturation, granule docking, and priming at the immunologic synapse (1). RAB27A is more restricted in expression than other Ras members; it is highly expressed only in melanocytes, hematopoietic cells, and other secretory cells (4). Defects in RAB27A are a cause of Griscelli syndrome type 2 (GS2), which results in an uncontrolled T-lymphocyte and macrophage activation syndrome (1).
Related Pathway

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WB Image
Western blot - Anti-RAB27A antibody [EPR3021]; All lanes : Anti-RAB27A [EPR3021] antibody at 1/1000 dilution.Lane 1 : HeLa cell Lysate.Lane 2 : MCF-7 cell Lysate.Lane 3 : HL-60 cell Lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 25 kDa.
IHC Image
Anti-RAB27A antibody [EPR3021]; Immunohistochemical staining of paraffin-embedded human tonsil tissue using anti- RAB27A.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-RAB27A [EPR3021] antibody; TA306983 stainingRAB27A inRhesus monkey fetal kidney cellsby ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed withformaldehyde and blocked with 10%serum for 1hour at 37°C. Samples were incubated with primary antibody (1/100 in 1% FCS/PBS) for 1 hour at 37°C. AnAlexa Fluor?568-conjugatedGoat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

 

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