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Anti-RAB27A Antibody EPR3021
Also for RAB27A (NM_183234)
|A synthetic peptide corresponding to residues in human RAB27A was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:100 - 1:500; ICC/IF: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse
|Homo sapiens RAB27A, member RAS oncogene family (RAB27A), transcript variant 2|
|GS2; HsT18676; RAB27; RAM|
|Ras-related protein (RAB27A), a member of the Ras family, is a GTP-binding protein that is involved in protein transport and small GTPase mediated signal transduction (1). RAB27A plays a role in cytotoxic granule exocytosis in lymphocytes (1). This process is mediated by RAB27A acting as an effector for JFC1/Slp1 and Munc13-4 (2). RAB27A also interacts with Slp4-a (synaptotagmin-like protein4-a) to negatively control dense-core vesicle exocytosis (3). In addition, it is required for granule maturation, granule docking, and priming at the immunologic synapse (1). RAB27A is more restricted in expression than other Ras members; it is highly expressed only in melanocytes, hematopoietic cells, and other secretory cells (4). Defects in RAB27A are a cause of Griscelli syndrome type 2 (GS2), which results in an uncontrolled T-lymphocyte and macrophage activation syndrome (1).|
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Western blot - Anti-RAB27A antibody [EPR3021]; All lanes : Anti-RAB27A [EPR3021] antibody at 1/1000 dilution.Lane 1 : HeLa cell Lysate.Lane 2 : MCF-7 cell Lysate.Lane 3 : HL-60 cell Lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 25 kDa.
Anti-RAB27A antibody [EPR3021]; Immunohistochemical staining of paraffin-embedded human tonsil tissue using anti- RAB27A.
Immunocytochemistry/ Immunofluorescence - Anti-RAB27A [EPR3021] antibody; ab108983 stainingRAB27A inRhesus monkey fetal kidney cellsby ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed withformaldehyde and blocked with 10%serum for 1hour at 37°C. Samples were incubated with primary antibody (1/100 in 1% FCS/PBS) for 1 hour at 37°C. AnAlexa Fluor?568-conjugatedGoat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.