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Anti-EGFR PHOSPHO Antibody EPR2257


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SKU Description Amount Price Availability*  
  • Rabbit monoclonal antibody against EGFR Phospho (pS695) (clone EPR2257 ) (Phospho-Specific)
100ul $325 3-7 Days
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues surrounding Serine 695 in human EGFR was used as an immunogen. The antibody detects EGFR only when phosphorylated on Serine 695.
Clone NameEPR2257 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF Suggested DilutionsICC: 1:100 - 250, WB: 1:1,000 - 5,000,
Predicted MW Explanation 134 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens epidermal growth factor receptor (EGFR), transcript variant 1
Alternative NameERBB; ERBB1; HER1; mENA; PIG61
Database LinkNP_005219
Entrez Gene 1956 Human
FunctionEpidermal growth factor receptor (EGFR) is a 175 kDa transmembrane glycoprotein receptor tyrosine kinase that is involved in diverse cellular functions including cell proliferation, differentiation, motility, survival and in tissue development (1, 2). EGFR is regulated by EGF-stimulated autophosphorylation and by phorbol ester-stimulated, protein kinase C (Ca2+/ phospholipid-dependent enzyme) mediated phosphorylation at identified sites. EGFR contains additional phosphorylation sites including a prominent phosphothreonine and several phosphoserines which account for the majority of phosphate covalently bound to the receptor in vivo. Analysis of predicted secondary structure of EGFR reveals that all of the phosphorylation sites are located near beta turns (3).
Related PathwayStem cell relevant signaling - JAK/STAT signaling pathwayProtein KinaseSecreted ProteinES Cell Differentiation/IPSTransmembraneAdult stem cellsCancer stem cellsDruggable Genome MAPK signaling pathwayErbB signaling pathwayCalcium signaling pathwayCytokine-cytokine receptor interactionEndocytosisDorso-ventral axis formationMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - EGFR (phospho S695) antibody [EPR2257]; All lanes : Anti-EGFR (phospho S695) antibody [EPR2257] at 1/1000 dilution.Lane 1 : A431 cell lysate untreated.Lane 2 : A431 cell lysate treated with EGF.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 134 kDa.
IF Image
Immunocytochemistry/ Immunofluorescence - EGFR (phospho S695) antibody [EPR2257]; Immunofluorescent staining of A431 cells using TA306976 at 1/100 dilution.


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