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Anti-EGFR PHOSPHO Antibody EPR2257
Also for EGFR (NM_005228)
|A synthetic peptide corresponding to residues surrounding Serine 695 in human EGFR was used as an immunogen. The antibody detects EGFR only when phosphorylated on Serine 695.|
||0.5~1.0 mg/ml (Lot Dependent)
||ICC: 1:100 - 250, WB: 1:1,000 - 5,000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens epidermal growth factor receptor (EGFR), transcript variant 1|
|ERBB; ERBB1; HER1; mENA; PIG61|
|Epidermal growth factor receptor (EGFR) is a 175 kDa transmembrane glycoprotein receptor tyrosine kinase that is involved in diverse cellular functions including cell proliferation, differentiation, motility, survival and in tissue development (1, 2). EGFR is regulated by EGF-stimulated autophosphorylation and by phorbol ester-stimulated, protein kinase C (Ca2+/ phospholipid-dependent enzyme) mediated phosphorylation at identified sites. EGFR contains additional phosphorylation sites including a prominent phosphothreonine and several phosphoserines which account for the majority of phosphate covalently bound to the receptor in vivo. Analysis of predicted secondary structure of EGFR reveals that all of the phosphorylation sites are located near beta turns (3). |
Cytokine-cytokine receptor interaction
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
MAPK signaling pathway
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Western blot - EGFR (phospho S695) antibody [EPR2257]; All lanes : Anti-EGFR (phospho S695) antibody [EPR2257] at 1/1000 dilution.Lane 1 : A431 cell lysate untreated.Lane 2 : A431 cell lysate treated with EGF.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 134 kDa.
Immunocytochemistry/ Immunofluorescence - EGFR (phospho S695) antibody [EPR2257]; Immunofluorescent staining of A431 cells using ab92629 at 1/100 dilution.