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Home Antibody All anti-EGFR antibodies

Anti-EGFR PHOSPHO Antibody EP774Y

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SKU Description Amount Price Availability*  
TA306975
  • Rabbit monoclonal antibody against Phospho-EGFR (pY1068)(clone EP774Y) (Phospho-Specific)
100ul 325 In Stock
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WB(1)
IHC(6)
IF(1)
Assay(1)
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Also for EGFR (NM_005228)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Tyr1068 of human EGF receptor was used as an immunogen.
Clone NameEP774Y IsotypeIgG
Species ReactivityHuman, Mouse Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, ASSAY, IHC, IF Suggested DilutionsIHC-FoFr: Use at an assay dependent dilution; WB: 1:500 - 1:1000; ICC/IF: 1:250 - 1:500; IHC-P: 1:250 - 1:500
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens epidermal growth factor receptor (EGFR), transcript variant 1
Alternative NameERBB; ERBB1; HER1; mENA; PIG61
Database LinkNP_005219
FunctionEpidermal growth factor receptor (EGFR) is a 170 kDa transmembrane glycoprotein receptor tyrosine kinase that, when activated by epidermal growth factor (EGF), affects cell growth and differentiation in normal and cancer cells (1). Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the receptor (2). The carboxy terminal tyrosine residues on EGFR, Tyr 1068, Tyr 1148, and Tyr 1173, are the major sites of autophosphorylation, which occurs as a result of EGF binding (3). EGFR overexpression is exhibited various cancer, such as glioma, colorectal carcinoma, breast carcinoma, and head and neck carcinoma (4). The phosphorylation level of EGFR is considered one of the important predicators for outcome in non-small cell lung cancer therapy (5).
Related Pathway
Adherens junction
Cytokine-cytokine receptor interaction
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
Focal Adhesion
MAPK signaling pathway

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WB Image
Western blot - Anti-EGFR (phospho Y1092) antibody [EP774Y]; All lanes : Anti-EGFR (phospho Y1092) antibody [EP774Y] at 1/50000 dilution.Lane 1 : A431 cell lysate with cells untreated with EGF.Lane 2 : A431 cell lysate with cells treated with EGF.Predicted band size : 170 kDa.Observed band size : 170 kDa.
Assay Image
Other-Anti-EGFR (phospho Y1092) antibody [EP774Y](TA306975); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Anti-EGFR (phospho Y1092) antibody [EP774Y]; Immunohistochemical staining of untreated (A) and Phosphatase- treated (B) paraffin-embedded breast adenocarcinoma tissue using TA306975.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (phospho Y1092) antibody [EP774Y]; TA306975 staining chondrocytes in tissue section (E13.5) of mouse limb, by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in paraformaldehyde, heat mediated antigen retrieval using 10mM Sodium Citrate buffer (pH 6.0) for 10 min at 95°C in a pressurized chamber and blocking in 5% serum for 1 hour at 25°C. Endogenous peroxidases were quenched with 3% H2o2 for 10 minutes. The primary antibody was diluted 1/250 in NGS buffer and incubated with sample for 8 hours at 4°C. A Biotin-conjugated goat polyclonal to rabbit IgG, diluted 1/500 was used as secondary. 1XTBS/0.1% Tween-20 (TBST) gave minimum background over 1XPBS as wash buffer.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (phospho Y1092) antibody [EP774Y]; TA306975 staining EGFR in adult mouse skin tissue by Immunohistochemistry (paraffin embedded sections). Tissue was fixed with paraformaldehyde, then a heat mediated antigen retrieval step was performed using citric buffer pH 6.0 and blocked with 10% serum for 1 hour at 20°C followed by incubation with the primary antibody, at a 1/200 dilution, for 12 hours at 4°C. Abcam biotinylated goat anti-rabbit IgG (H+L) (ready to use) was used as the secondary antibody, catalogue number .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (phospho Y1092) antibody [EP774Y]; TA306975 showing positive staining in Papillary carcinoma of thyroid gland tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (phospho Y1092) antibody [EP774Y]; TA306975 showing positive staining in Glioma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (phospho Y1092) antibody [EP774Y]; TA306975 showing positive staining in Cervical carcinoma tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1092) antibody [EP774Y]; Immunofluorescent staining of untreated (C) and Phosphatase- treated (D) A431 cells using TA306975.

 

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