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Anti-ERCC1 Antibody EP2143Y
Also for ERCC1 (NM_202001)
|A synthetic peptide corresponding to N-terminus of human ERCC1 was used. Might also detect heterodimer composed of ERCC1 and XPF/ERCC4 at 100 -150 kDa.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:5000; FC: 1:30
|Does not react with Mouse, Rat. Is unsuitable for ICC,IHC-P or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for ICC,IHC-P or IP.
|Homo sapiens excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence) (ERCC1), transcript variant 1|
|COFS4; RAD10; UV20|
|Excision Repair Cross Complementing 1 (ERCC1) is a mammalian nucleotide excision repair (NER) enzyme involved in repair of damaged DNA. ERCC1 is a homologous to RAD10 in Saccharomyces cerevisiae which is required in mitotic intrachromosomal recombination and repair. Specifically, during DNA repair, ERCC1 forms a complex with XPF/ERCC4 which makes incision 5’ to the lesion site (1). ERCC1 is required in repair of cisplatin-induced DNA adducts and ultraviolet (UV)-induced DNA damage. A defect in ERCC1 can lead to Cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) and UV-induced skin cancer (2).|
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Western blot - ERCC1 antibody [EP2143Y]; Anti-ERCC1 antibody [EP2143Y] at 1/2000 dilution + SKBR-3 cell lysate at 10 µg.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 32 kDa.Observed band size : 36 kDa .Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.