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Anti-WT1 Antibody CAN-R9(IHC)-56-2
|A recombinant protein corresponding to the N-terminus of human WT was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||IHC-Fr: 1:250; FC: 1:100; WB: 1:1000 - 1:5000; IHC-P: 1:100 - 1:250; ICC/IF: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens Wilms tumor 1 (WT1), transcript variant A|
|AWT1; EWS-WT1; GUD; NPHS4; WAGR; WIT-2; WT33|
|Wilms Tumor (WT) is a 55 kDa protein thought to play a major role in various cancers and developmental disorders, commonly kidney tumors and glomerular diseases. WT is a zinc finger transcription factor that is mutated in Wilms tumors and highly expressed in a wide variety of other malignancies (1). WT1 consists of two major isoforms; WT1 (-KTS), a transcription factor, and WT1 (+KTS) (2). Mutations in the zinc finger regions of WT are detected in nearly all patients of Denys-Drash syndrome and in some patients with isolated diffuse mesangial sclerosis. Also, mutations leading to the loss of the transcription factor isoform have been observed in all patients with Frasier syndrome.|
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Western blot - Wilms Tumor Protein antibody; Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] at 1/1000 dilution + Cell lysates prepared from Ramos cells.Predicted band size : 55 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Wilms Tumor Protein antibody; ab89901 at 1/250 dilution staining Wilms Tumor Protein in human fetal tissue sections by Immunohistochemistry (Formalin fixed Paraffin-embedded tissue sections).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Wilms Tumor Protein antibody; ab89901 at 1/250 dilution staining Wilms Tumor Protein in human Wilms tumor tissue section by IHC-P.
Flow Cytometry-Anti-Wilms Tumor Protein antibody(ab89901); Overlay histogram showing K562 cells stained with ab89901 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.