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Home Antibody All anti-DLG4 antibodies

Anti-DLG4 Antibody EP2652Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303851
  • Rabbit monoclonal antibody against PSD95 (EP2652Y )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419977) , 20ug Explanation
100ul 325 In Stock
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WB(1)
FC(1)
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Also for DLG4 (NM_001365)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the N-terminus of human PSD95 was used as an immunogen.
Clone NameEP2652Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, FC Suggested DilutionsICC/IF: Use at an assay dependent concentration; WB: 1:1000 - 1:5000; IP: 1:10; ICC: 1:100 - 1:250; FC: 1:20
Predicted MW Explanation 95 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IHC-P.

Reference Data

Target NameHomo sapiens discs, large homolog 4 (Drosophila) (DLG4), transcript variant 1
Alternative NamePSD95; SAP-90; SAP90
Database LinkNP_001356
Entrez Gene 1742 Human
Entrez Gene 13385 Mouse
Entrez Gene 29495 Rat
FunctionThis gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Related PathwayDruggable Genome Huntington's disease

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - PSD95 antibody [EP2652Y]; All lanes : Anti-PSD95 antibody [EP2652Y] at 1/2000 dilution.Lane 1 : Human brain tissue lysate.Lane 2 : Mouse brain tissue lysate.Lane 3 : Rat brain tissue lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 95 kDa.Observed band size : 95 kDa.
FC Image
Flow Cytometry-Anti-PSD95 antibody [EP2652Y](TA303851); Overlay histogram showing SH-SY5Y cells stained with TA303851 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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