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Home Antibody All anti-HLA antibodies

Anti-HLA Antibody EP1395Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303840
  • Rabbit monoclonal antibody against MHC class 1 (EP1395Y )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400771) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for HLA (NM_002116)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human MHC class 1 was used as an immunogen
Clone NameEP1395Y IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsIHC-Fr: Use at an assay dependent concentration; WB: 1:10000 - 1:50000; IP: 1:30; FC: 1:10 - 1:100; IHC-P: 1:250 - 1:500; ICC/IF: 1:250 - 1:500
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens major histocompatibility complex, class I, A (HLA-A), transcript variant 1 (A*03:01:0:01 allele)
Alternative NameHLAA
Database LinkNP_002107
Entrez Gene 3105 Human
FunctionHLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq].
Related PathwayTransmembrane EndocytosisCell adhesion molecules (CAMs)Antigen processing and presentationNatural killer cell mediated cytotoxicityType I diabetes mellitusAutoimmune thyroid diseaseMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-HLA A [EP1395Y] antibody; Anti-HLA A [EP1395Y] antibody at 1/10000 dilution + Raji cell lysate at 10 ug.Secondary.Goat anti rabbit IgG HRP conjugated at 1/2000 dilution.Predicted band size : 41 kDa.Observed band size : 41 kDa.
IHC Image
Anti-HLA A [EP1395Y] antibody; Ab52922 at 1/250 dilution staining human tonsil; paraffin embedded.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-HLA A [EP1395Y] antibody; ICC/IF image of TA303840 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
FC Image
Flow Cytometry - Anti-HLA A [EP1395Y] antibody; Overlay histogram showing Raji cells stained with TA303840 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Raji cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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