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Anti-MAPK3 PHOSPHO Antibody E337
Also for MAPK3 (NM_002746)
|A synthetic phospho-peptide corresponding to residues surrounding Thr202 and Tyr204 of human ERK1 was used as immunogen. The antibody only detects ERK1 phosphorylated on Threonine 202 and Tyrosine 204, or ERK2 phosphorylated on Threonine 185 and Tyrosine|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||WB: 1:500; IHC: 1:50; ICC: 1:250
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Does not react with Mouse, Rat
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Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) [E337] antibody; .developed using the ECL technique.Performed under reducing conditions.Predicted band size : 42 , 44 kDa.Exposure time : 16 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) [E337] antibody; Immunohistochemical analysis of paraffin-embedded human thyroid gland cancer using anti-ERK1(pT202/pY204)/ERK2(pT185/pY187) diluted 1:50
Flow Cytometry - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) [E337] antibody; Overlay histogram showing HeLa cells stained with TA303838 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.