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Anti-PPP1R12A Antibody YE336
Also for PPP1R12A (NM_002480)
|A synthetic peptide corresponding to residues in C-terminus of human MYPT1 or MYPT2 was used as immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IF, FC
||WB: 1:5000; ICC/IF: 1:100; FC: 1:100; IP: 1:80
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|IgG fraction (Protein A or G Sepharose)
|Is unsuitable for IHC.
|Homo sapiens protein phosphatase 1, regulatory subunit 12A (PPP1R12A), transcript variant 1|
|M130; MBS; MYPT1|
|Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. |
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Western blot - Myosin Phosphatase 1 / Myosin Phosphatase 2 antibody [YE336]; Anti-Myosin Phosphatase 1+Myosin Phosphatase 2 antibody [YE336] at 1/5000 dilution + 293T cell lysate.Predicted band size : 110 kDa.Observed band size : 110 kDa.
Immunocytochemistry/ Immunofluorescence - Myosin Phosphatase 1 / Myosin Phosphatase 2 antibody [YE336]; ab32519 at a 1:100 dilution staining Myosin Phosphatase 1 / Myosin Phosphatase 2 in 293 cells using Immunocytochemistry/Immunofluorescence.
Immunocytochemistry/ Immunofluorescence - Myosin Phosphatase 1 / Myosin Phosphatase 2 antibody [YE336]; ab32519 staini
Flow Cytometry - Myosin Phosphatase 1+Myosin Phosphatase 2 antibody [YE336]; Overlay histogram showing HEK293 cells stained with ab32519 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.