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Home Antibody All anti-HIST1H3F antibodies

Anti-HIST1H3F Antibody EPR996Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303820
  • Rabbit monoclonal antibody against Histone H3 Acetyl (K56) (EPR996Y )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC412143) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(5)
IF(2)
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Also for HIST1H3F (NM_021018)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenA synthetic acetylated peptide corresponding to residues surrounding Lys 56 of Histone H3 was used as immunogen. The antibody only detects Histone H3 acetylated on Lysine 56.
Clone NameEPR996Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000 - 2000; IHC: 1:100 250; ICC: 1:100 250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens histone cluster 1, H3f (HIST1H3F)
Alternative NameH3/i; H3FI
Database LinkNP_066298
FunctionHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Histone H3 (acetyl K56) antibody [EPR996Y]; All lanes : Anti-Histone H3 (acetyl K56) antibody [EPR996Y] at 1/2000 dilution.Lane 1 : C6 cell lysate, untreated.Lane 2 : C6 cell lysate, treated with TSA.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/1000 dilution.Predicted band size : 15 kDa.Observed band size : 15 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (acetyl K56) antibody [EPR996Y]; IHC image of TA303820 staining in Breast Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with TA303820, undiluted, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Histone H3 (acetyl K56) antibody [EPR996Y](TA303820); TA303820 showing positive staining in Cervical carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Histone H3 (acetyl K56) antibody [EPR996Y](TA303820); TA303820 showing positive staining in Gastric adenocarcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Histone H3 (acetyl K56) antibody [EPR996Y](TA303820); TA303820 showing positive staining in Lung adenocarcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Histone H3 (acetyl K56) antibody [EPR996Y](TA303820); TA303820 showing positive staining in Lymphoma tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K56) antibody [EPR996Y]; TA303820 stainingHistoneH3 (HEK56 Ac)inAfricanGreen Monkey kidney epithelial (CV-1 line) cellby ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed withparaformaldehyde, permeabilized with Triton and blocked with 5% BSA for 1hour at 21°C. Samples were incubated with primary antibody (1/500) for 16 hour at 4°C.CV-1 cells were infected with Simian virus 40 (SV40). 32 hours later the cells were incubated with EdU for30 minutes before pre-extraction to detect active DNA replication foci and fixed, stained, and visualized by confocal microscopy.Blue - Hoechst stain, red - anti-T-Ag, magenta -EdU, green -anti-H3K56 Ac. Bar, 5 um.
IF Image
Immunocytochemistry/ Immunofluorescence - Histone H3 (acetyl K56) antibody [EPR996Y]; TA303820 at 1/100 dilution staining Histone H3 in HeLa cells by Immunofluorescence.

 

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