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Home Antibody All anti-HIST2H2BF antibodies

Anti-HIST2H2BF Antibody EP957Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303813
  • Rabbit monoclonal antibody against Histone H2B(EP957Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC422516) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for HIST2H2BF (NM_001024599)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic acetylated peptide corresponding to residues surrounding Histone H2B was used as an immunogen.
Clone NameEP957Y IsotypeIgG
Species ReactivityMouse, Rat, Cow, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:20000; IP: 1:30; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:50 - 1:100; FC: 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens histone cluster 2, H2bf (HIST2H2BF), transcript variant 1
Alternative NameFLJ35099; FLJ56780; FLJ56787; MGC131639
Database LinkNP_001019770
Entrez Gene 440689 Human
Function
Related Pathway Systemic lupus erythematosus

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Histone H2B antibody [EP957Y]; Anti-Histone H2B antibody [EP957Y] at 1/20000 dilution + A431 cell lysate at 10 ug.Secondary.HRP-labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 14 kDa.Observed band size : 14 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Histone H2B antibody [EP957Y]; Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using TA303813 at a dilution of 1/250-1/500.
IF Image
Immunocytochemistry/ Immunofluorescence - Histone H2B antibody [EP957Y]; Fluorescent staining of HeLa cells using TA303813 at a dilution of 1/50-1/100.
FC Image
Flow Cytometry - Anti-Histone H2B antibody [EP957Y]; Overlay histogram showing HeLa cells stained with TA303813 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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