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Home Antibody All anti-HIST2H2BF antibodies

Anti-HIST2H2BF Antibody EP957Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303813
  • Rabbit monoclonal antibody against Histone H2B(EP957Y)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC422516) , 20ug
100ul $325 3-7 Days
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for HIST2H2BF (NM_001024599)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic acetylated peptide corresponding to residues surrounding Histone H2B was used as an immunogen.
Clone NameEP957Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:20000; IP: 1:30; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:50 - 1:100; FC: 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens histone cluster 2, H2bf (HIST2H2BF), transcript variant 1
Alternative NameFLJ35099; FLJ56780; FLJ56787; MGC131639
Database LinkNP_001019770
Function
Related Pathway

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WB Image
Western blot - Histone H2B antibody [EP957Y]; Anti-Histone H2B antibody [EP957Y] at 1/20000 dilution + A431 cell lysate at 10 µg.Secondary.HRP-labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 14 kDa.Observed band size : 14 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Histone H2B antibody [EP957Y]; Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using TA303813 at a dilution of 1/250-1/500.
IF Image
Immunocytochemistry/ Immunofluorescence - Histone H2B antibody [EP957Y]; Fluorescent staining of HeLa cells using TA303813 at a dilution of 1/50-1/100.
FC Image
Flow Cytometry - Anti-Histone H2B antibody [EP957Y]; Overlay histogram showing HeLa cells stained with TA303813 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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