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Home Antibody All anti-HIST2H2BF antibodies

Anti-HIST2H2BF Antibody EP959Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303809
  • Rabbit monoclonal antibody against Histone H3, Acetyl (K18)(EP959Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC422516) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for HIST2H2BF (NM_001024599)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic acetylated peptide corresponding to residues surrounding Lys18 of Histone H3 was used as immunogen. The antibody only detects Histone H3 acetylated on Lysine 18.
Clone NameEP959Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsICC/IF: 1:100 - 1:250; WB: 1:500; IHC-P: Use at an assay dependent concentration; FC: 1:100; ChIP: Use at an assay dependent concentration
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens histone cluster 2, H2bf (HIST2H2BF), transcript variant 1
Alternative NameFLJ35099; FLJ56780; FLJ56787; MGC131639
Database LinkNP_001019770
Function
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Histone H3 antibody [EP959Y]; All lanes : Anti-Histone H3 (acetyl K18) antibody [EP959Y] at 1/50000 dilution.Lane 1 : C6 cell lysate, untreated.Lane 2 : C6 cell lysate, treated with TSA.Lysates/proteins at 10 µg per lane.Predicted band size : 17 kDa.Observed band size : 17 kDa.Additional bands at : 50 kDa (possible non-specific binding),60 kDa (possible non-specific binding).
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Histone H3 antibody [EP959Y]; TA303809 diluted 1:100, staining acetylated histone H3 on human breast carcinoma sections.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (acetyl K18) antibody [EP959Y]; TA303809 (1/500) staining Histone H3 (acetyl K18) in asynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.
FC Image
Flow Cytometry - Anti-Histone H3 (acetyl K18) antibody [EP959Y]; Overlay histogram showing HeLa cells stained with TA303809 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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