OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-HIST1H2AA antibodies

Anti-HIST1H2AA Antibody EP856Y

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303803
  • Rabbit monoclonal antibody against Histone H2A, Acetyl-K5(EP856Y)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC406865) , 20ug
100ul 325 3-7 Days
Add to Shopping Cart
WB(1)
IHC(1)
IF(1)
spacer
Also for HIST1H2AA (NM_170745)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human Histone H2A was used as an immunogen.
Clone NameEP856Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:100,000; IHC: 1:250 500; ICC: 1:500 1000; IP: 1:70
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens histone cluster 1, H2aa (HIST1H2AA)
Alternative NamebA317E16.2; H2AA; H2AFR
Database LinkNP_734466
FunctionHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene contain a palindromic termination element.
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Histone H2A (acetyl K5) antibody [EP856Y]; All lanes : Anti-Histone H2A (acetyl K5) antibody [EP856Y] at 1/100000 dilution.Lane 1 : C6 cell lysate.Lane 2 : C6 + TSA membrane.Predicted band size : 14 kDa.Observed band size : 14 kDa.Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Histone H2A (acetyl K5) antibody [EP856Y]; Ab45152 staining human Histone H2A in human breast carcinoma by immunohistochemistry using paraffin embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence-Histone H2A (acetyl K5) antibody [EP856Y](TA303803); ICC/IF image of TA303803 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title