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Home Antibody All anti-HIST1H2AA antibodies

Anti-HIST1H2AA Antibody EP856Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303803
  • Rabbit monoclonal antibody against Histone H2A, Acetyl-K5(EP856Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC406865) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)
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Also for HIST1H2AA (NM_170745)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human Histone H2A was used as an immunogen.
Clone NameEP856Y IsotypeIgG
Species ReactivityMouse, Rat, Human, Caenorhabditis elegans ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:100,000; IHC: 1:250 500; ICC: 1:500 1000; IP: 1:70
Predicted MW Explanation 14 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens histone cluster 1, H2aa (HIST1H2AA)
Alternative NamebA317E16.2; H2AA; H2AFR
Database LinkNP_734466
Entrez Gene 221613 Human
Entrez Gene 319163 Mouse
Entrez Gene 24828 Rat
FunctionHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene contain a palindromic termination element.
Related Pathway Systemic lupus erythematosus

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Histone H2A (acetyl K5) antibody [EP856Y]; All lanes : Anti-Histone H2A (acetyl K5) antibody [EP856Y] at 1/100000 dilution.Lane 1 : C6 cell lysate.Lane 2 : C6 + TSA membrane.Predicted band size : 14 kDa.Observed band size : 14 kDa.Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Histone H2A (acetyl K5) antibody [EP856Y]; Ab45152 staining human Histone H2A in human breast carcinoma by immunohistochemistry using paraffin embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence-Histone H2A (acetyl K5) antibody [EP856Y](TA303803); ICC/IF image of TA303803 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.

 

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