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Anti-HIST1H2AA Antibody EP856Y
Also for HIST1H2AA (NM_170745)
|A synthetic peptide corresponding to residues near the N-terminus of human Histone H2A was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:100,000; IHC: 1:250 500; ICC: 1:500 1000; IP: 1:70
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens histone cluster 1, H2aa (HIST1H2AA)|
|bA317E16.2; H2AA; H2AFR|
|Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene contain a palindromic termination element. |
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Western blot - Histone H2A (acetyl K5) antibody [EP856Y]; All lanes : Anti-Histone H2A (acetyl K5) antibody [EP856Y] at 1/100000 dilution.Lane 1 : C6 cell lysate.Lane 2 : C6 + TSA membrane.Predicted band size : 14 kDa.Observed band size : 14 kDa.Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Paraffin-embedded sections) - Histone H2A (acetyl K5) antibody [EP856Y]; Ab45152 staining human Histone H2A in human breast carcinoma by immunohistochemistry using paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence-Histone H2A (acetyl K5) antibody [EP856Y](ab45152); ICC/IF image of ab45152 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.