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Anti-RAPTOR Antibody EP539Y
Also for RAPTOR (NM_020761)
|A synthetic peptide corresponding to residues near the C-terminus of human Raptor was used as immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1:500; IP: 1:30; FC: 1:30; ICC/IF: 1:100 - 1:250; IHC-P: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens regulatory associated protein of MTOR, complex 1 (RPTOR), transcript variant 1|
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Western blot - Raptor antibody [EP539Y]; All lanes : Anti-Raptor antibody [EP539Y] at 1/500 dilution.Lane 1 : 293 cell lysate.Lane 2 : MCF-7 cell lysate.Lane 3 : HeLa cell lysate.Lane 4 : A431 cell lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 149 kDa.Observed band size : 140 kDa .Additional bands at : 21 kDa,37 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Paraffin-embedded sections) - Raptor antibody [EP539Y]; Immunohistochemical analysis of paraffin-embedded human colon using anti-Raptor TA303799 at 1/50 dilution.