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Anti-DIABLO Antibody Y12


Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
  • Rabbit monoclonal antibody against Smac/Diablo(E12)
100ul $325 3-7 Days
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Also for DIABLO (NM_138929)
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OriGene Data

ImmunogenA synthetic peptide corresponding to C-terminal residues human Smac/DIABLO was used as immunogen.
Clone NameY12 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:1000; IHC-P: Use at an assay dependent dilution; ICC: 1:10; IP: 1:10 - 1:80; FC: 1:10 - 1:50; ICC/IF: Use at an assay dependent concentration
Predicted MW Explanation 27 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Protein A purified

Reference Data

Target NameHomo sapiens diablo, IAP-binding mitochondrial protein (DIABLO), nuclear gene encoding mitochondrial protein, transcript variant 3
Alternative NameDFNA64; DIABLO-S; SMAC; SMAC3
Database LinkNP_620307
Entrez Gene 56616 Human
Entrez Gene 66593 Mouse
Entrez Gene 288753 Rat
FunctionThis gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Four alternatively spliced transcript variants have been described for this gene, with two of them encoding different isoforms and the other two probably not encoding a protein. [provided by RefSeq].
Related PathwayTransmembrane

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Smac / Diablo antibody [Y12]; Anti-Smac / Diablo antibody [Y12] at 1/1000 dilution + Jurkat cell lysate.Predicted band size : 27 kDa.Observed band size : 21 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Smac / Diablo antibody [Y12]; TA303797, at a 1/10 dilution, staining human Smac/Diablo in skin cancer tissue by Immunohistochemistry, Paraffin embedded tissue
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Smac / Diablo antibody [Y12]; Immunofluorescence analysis of IMR32 cells, staining Smac / Diablo with TA303797. Cells were fixed with 4% paraformaldehyde before incubating with primary antibody. An AlexaFluor?-conjugated anti-rabbit IgG was used as the secondary antibody.


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