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Home Antibody All anti-RANBP9 antibodies

Anti-RANBP9 Antibody EP1353Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303794
  • Rabbit monoclonal antibody against RanBP9 (C-term) (EP1353Y )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401682) , 20ug
100ul 325 In Stock
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WB(1)
IHC(1)
FC(1)
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Also for RANBP9 (NM_005493)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the C-terminus of human RANBP9 was used as an immunogen.
Clone NameEP1353Y IsotypeIgG
Species ReactivityMouse, Human (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:1000; IP: 1:20; FC: 1:1000; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Rat

Reference Data

Target NameHomo sapiens RAN binding protein 9 (RANBP9)
Alternative NameBPM-L; BPM90; RanBP7; RANBPM
Database LinkNP_005484
FunctionThis gene encodes a protein that binds RAN, a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The protein encoded by this gene has also been shown to interact with several other proteins, including met proto-oncogene, homeodomain interacting protein kinase 2, androgen receptor, and cyclin-dependent kinase 11. [provided by RefSeq].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - RanBP9 antibody [EP1353Y]; Anti-Importin 9 antibody [EP1353Y] at 1/1000 dilution + Fetal brain lysate at 10 ug.Secondary.HRP-labelled goat anti-rabbit. at 1/2000 dilution.Predicted band size : 115 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - RanBP9 antibody [EP1353Y]; Immunohistochemical staining of paraffin-embedded human lymphoma using TA303794 at a dilution of 1/50.
FC Image
Flow Cytometry - Anti-Importin 9 antibody [EP1353Y]; Overlay histogram showing HeLa cells stained with TA303794 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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