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Anti-MST4 Antibody EP1864Y
Also for MST4 (NM_016542)
|A synthetic peptide corresponding to residues on the N-terminus of human Mst4 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:100000 - 1:200000; IP: 1:50; ICC: 1:100 - 1:250; FC: 1:80; IHC-P: Use at an assay dependent dilution
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens serine/threonine protein kinase MST4 (MST4), transcript variant 1|
|The product of this gene is a member of the GCK group III family of kinases, which are a subset of the Ste20-like kinases. The encoded protein contains an amino-terminal kinase domain, and a carboxy-terminal regulatory domain that mediates homodimerization. The protein kinase localizes to the Golgi apparatus and is specifically activated by binding to the Golgi matrix protein GM130. It is also cleaved by caspase-3 in vitro, and may function in the apoptotic pathway. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]. |
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Western blot - MST4 antibody [EP1864Y]; Anti-MST4 antibody [EP1864Y] at 1/200000 dilution + HeLa cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 47 kDa.Observed band size : 50 kDa .
Western blot - Anti-MST4 antibody [EP1864Y]; Anti-MST4 antibody [EP1864Y] at 1/20000 dilution + Active human MST4 full length protein at 0.01 µg.Secondary.Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution.Exposure time : 20 seconds
Immunohistochemistry (Paraffin-embedded sections) - MST4 antibody [EP1864Y]; Immunohistochemical analysis of paraffin-embedded human placenta using ab52491 at a 1/100 dilution.