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Home Antibody All anti-SIRT1 antibodies

Anti-SIRT1 PHOSPHO Antibody EP2850Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303786
  • Rabbit monoclonal antibody against Sir2/SIRT1 Phospho (pS27) (EP2850Y ) (phospho-specific)
100ul 325 3-7 Days
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Also for SIRT1 (NM_012238)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA phospho-specific peptide corresponding to residues surrounding serine 27 of human Sir2/SIRT1 was used as an immunogen. This antibody detects Sir2/SIRT1 phosphorylated on serine 27.
Clone NameEP2850Y IsotypeTissue culture supernatant
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB Suggested DilutionsWB: 1:5000 - 1:10000; IP: 1:20
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt,ICC or IHC-P.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt,ICC or IHC-P.

Reference Data

Target NameHomo sapiens sirtuin 1 (SIRT1), transcript variant 1
Alternative NameSIR2L1
Database LinkNP_036370
FunctionThis gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq].
Related Pathway

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WB Image
Western blot - SIRT1 (phospho S27) antibody [EP2850Y]; All lanes : Anti-SIRT1 (phospho S27) antibody [EP2850Y] at 1/10000 dilution.Lane 1 : 293T cell lysates, untreated.Lane 2 : 293T cell lysates, treated with Calyculin A.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 82 kDa.Observed band size : 110 kDa .</title><style>.an6q{position:absolute;clip:rect(487px,auto,auto,487px);}</style><div class=an6q>According to your current <a href=http://paydayloansforsure.com >need cash now</a> you really look at.</div>

 

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