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Anti-SIRT1 PHOSPHO Antibody EP2850Y
Also for SIRT1 (NM_012238)
|A phospho-specific peptide corresponding to residues surrounding serine 27 of human Sir2/SIRT1 was used as an immunogen. This antibody detects Sir2/SIRT1 phosphorylated on serine 27.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:5000 - 1:10000; IP: 1:20
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt,ICC or IHC-P.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,ICC or IHC-P.
|Homo sapiens sirtuin 1 (SIRT1), transcript variant 1|
|This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq]. |
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Western blot - SIRT1 (phospho S27) antibody [EP2850Y]; All lanes : Anti-SIRT1 (phospho S27) antibody [EP2850Y] at 1/10000 dilution.Lane 1 : 293T cell lysates, untreated.Lane 2 : 293T cell lysates, treated with Calyculin A.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 82 kDa.Observed band size : 110 kDa .