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Anti-MAP3K7IP1 Antibody EPR635Y
Also for MAP3K7IP1 (NM_006116)
|A synthetic peptide corresponding to residues near the C-term of human TAB1 was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||WB: 1:1000 2000; IHC: 1:100 250; ICC: 1:50 100; FC: 1:40; IP: 1:30
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens TGF-beta activated kinase 1/MAP3K7 binding protein 1 (TAB1), transcript variant alpha|
|3 -Tab1; MAP3K7IP1|
Entrez Gene 10454 Human
Entrez Gene 66513 Mouse
Entrez Gene 315139 Rat
|The protein encoded by this gene was identified as a regulator of the MAP kinase kinase kinase MAP3K7/TAK1, which is known to mediate various intracellular signaling pathways, such as those induced by TGF beta, interleukin 1, and WNT-1. This protein interacts and thus activates TAK1 kinase. It has been shown that the C-terminal portion of this protein is sufficient for binding and activation of TAK1, while a portion of the N-terminus acts as a dominant-negative inhibitor of TGF beta, suggesting that this protein may function as a mediator between TGF beta receptors and TAK1. This protein can also interact with and activate the mitogen-activated protein kinase 14 (MAPK14/p38alpha), and thus represents an alternative activation pathway, in addition to the MAPKK pathways, which contributes to the biological responses of MAPK14 to various stimuli. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]. |
|Druggable Genome MAPK signaling pathwayToll-like receptor signaling pathwayNOD-like receptor signaling pathway|
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Western blot - TAB1 antibody [EPR635Y]; Anti-TAB1 antibody [EPR635Y] at 1/2000 dilution + MCF-7 cell lysate at 10 ug.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 55 kDa.Observed band size : 55 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TAB1 antibody [EPR635Y]; Immunohistochemical staining of TAB1 in paraffin embedded human tonsils using TA303773 at a 1/100 dilution.
Flow Cytometry - Anti-TAB1 antibody [EPR635Y]; Overlay histogram showing HeLa cells stained with TA303773 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.