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Home Antibody All anti-MAP3K7IP1 antibodies

Anti-MAP3K7IP1 Antibody EPR635Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303773
  • Rabbit monoclonal antibody against TAB1 (EPR635Y )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC416846) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(1)
FC(1)
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Also for MAP3K7IP1 (NM_006116)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-term of human TAB1 was used as an immunogen.
Clone NameEPR635Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:1000 2000; IHC: 1:100 250; ICC: 1:50 100; FC: 1:40; IP: 1:30
Predicted MW Explanation 55 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens TGF-beta activated kinase 1/MAP3K7 binding protein 1 (TAB1), transcript variant alpha
Alternative Name3 -Tab1; MAP3K7IP1
Database LinkNP_006107
Entrez Gene 10454 Human
Entrez Gene 66513 Mouse
Entrez Gene 315139 Rat
FunctionThe protein encoded by this gene was identified as a regulator of the MAP kinase kinase kinase MAP3K7/TAK1, which is known to mediate various intracellular signaling pathways, such as those induced by TGF beta, interleukin 1, and WNT-1. This protein interacts and thus activates TAK1 kinase. It has been shown that the C-terminal portion of this protein is sufficient for binding and activation of TAK1, while a portion of the N-terminus acts as a dominant-negative inhibitor of TGF beta, suggesting that this protein may function as a mediator between TGF beta receptors and TAK1. This protein can also interact with and activate the mitogen-activated protein kinase 14 (MAPK14/p38alpha), and thus represents an alternative activation pathway, in addition to the MAPKK pathways, which contributes to the biological responses of MAPK14 to various stimuli. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq].
Related PathwayDruggable Genome MAPK signaling pathwayToll-like receptor signaling pathwayNOD-like receptor signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - TAB1 antibody [EPR635Y]; Anti-TAB1 antibody [EPR635Y] at 1/2000 dilution + MCF-7 cell lysate at 10 ug.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 55 kDa.Observed band size : 55 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TAB1 antibody [EPR635Y]; Immunohistochemical staining of TAB1 in paraffin embedded human tonsils using TA303773 at a 1/100 dilution.
FC Image
Flow Cytometry - Anti-TAB1 antibody [EPR635Y]; Overlay histogram showing HeLa cells stained with TA303773 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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