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Anti-MAPKAPK2 PHOSPHO Antibody EPR992Y
Also for MAPKAPK2 (NM_004759)
|A synthetic peptide corresponding to residues near C-terminus of human MAPKKAP Kinase-2 was used as immunogen.|
||Lot dependent; please refer to CoA along with shipment
||WB: 1:500; IHC: 1:100 - 250; ICC: 1:100 - 250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Does not react with Mouse, Rat
|Homo sapiens mitogen-activated protein kinase-activated protein kinase 2 (MAPKAPK2), transcript variant 1|
|MAPKAP-K2; MK-2; MK2|
|This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]. |
MAPK signaling pathway
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Western blot - MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y]; All lanes : Anti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] at 1/500 dilution.Lane 1 : HeLa cell lysate, untreated.Lane 2 : HeLa cell lysate treated with anisomycin.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 46 kDa.Observed band size : 50 kDa .
Western blot - Anti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y]; All lanes : Anti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y].Lane 1 : HEK293 cells transfected with GFP-MK2 .Lane 2 : HEK293 cells transfected with GFP-MK2 and treated 1h with p38 inhibitor.Lane 3 : HEK293 cells transfected with GFP-T334A MK2.Predicted band size : 46 kDa.
Immunohistochemistry (Paraffin-embedded sections) - MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y]; Ab51018 (1/100) staining human MAPKAP kinase 2 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.