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Anti-MAPKAPK2 Antibody E341
Also for MAPKAPK2 (NM_004759)
|A synthetic peptide corresponding to residues near C-terminus of human MAPKKAP Kinase-2 was used as immunogen.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
||WB: 1:500-1,000; IP: 1:200
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Homo sapiens mitogen-activated protein kinase-activated protein kinase 2 (MAPKAPK2), transcript variant 1|
|MAPKAP-K2; MK-2; MK2|
|This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]. |
MAPK signaling pathway
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Western blot - MAPKAP Kinase 2 antibody [E341]; Anti-MAPKAP Kinase 2 antibody [E341] at 1/1000 dilution + Hela cell lysate.Predicted band size : 46 kDa.Observed band size : 46 kDa.
Western blot - Anti-MAPKAP Kinase 2 antibody [E341]; Lanes 1 - 2 : Anti-MAPKAP Kinase 2 (phospho T334) antibody [EPR992Y] at 1/500 dilution.Lanes 3 - 4 : Anti-MAPKAP Kinase 2 antibody [E341] at 1/500 dilution.Lane 1 : UV treated HeLa cells.Lane 2 : UV treated HeLa cells + p38 inhibitor.Lane 3 : UV treated HeLa cells.Lane 4 : UV treated HeLa cells + p38 inhibitor.Secondary.IRDye? 680 LICOR goat anti-rabbit.Performed under reducing conditions.Predicted band size : 46 kDa.Observed band size : 48 kDa .Additional bands at : 55 kDa (possible cross reactivity).Exposure time : 1 minute