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Anti-PRC1 PHOSPHO Antibody EP1514Y
Also for PRC1 (NM_199414)
|A phospho specific peptide corresponding to residues surrounding Threonine 481 of human PRC1 was used as an immunogen. This antibody detects PRC1 phosphorylated at Theonine 481.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:500; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens protein regulator of cytokinesis 1 (PRC1), transcript variant 3|
|This gene encodes a protein that is involved in cytokinesis. The encoded protein is at high level during S and G2/M and drop dramatically after cell exit mitosis and enter G1. It is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). At least three alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]. |
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Western blot - PRC1 (phospho T481) antibody [EP1514Y]; All lanes : Anti-PRC1 (phospho T481) antibody [EP1514Y] at 1/500 dilution.Lane 1 : HeLa cell lysates (untreated).Lane 2 : HeLa cell lysates treated with aphidicolin + nocodazole.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP labeled, at 1/2000 dilution.Predicted band size : 72 kDa.Observed band size : 72 kDa.
Immunocytochemistry - PRC1 (phospho T481) antibody [EP1514Y]; ab62366 at 1/100 dilution staining PRC1 in HeLa cells by Immunocytochemistry.