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Anti-DYNLL1 Antibody EP1660Y
Also for DYNLL1 (NM_001037494)
|A synthetic peptide corresponding to residues on the N-terminus of human DLC8 was used as an immunogen.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, IP
||WB: 1:1000 - 1:10000; IHC-P: Use at an assay dependent dilution; ICC: 1:100 - 1:250; IP: 1:100; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt.
|Homo sapiens dynein, light chain, LC8-type 1 (DYNLL1), transcript variant 1|
|DLC1; DLC8; DNCL1; DNCLC1; hdlc1; LC8; LC8a; PIN|
|Cytoplasmic dyneins are large enzyme complexes with a molecular mass of about 1,200 kD. They contain two force-producing heads formed primarily from dynein heavy chains, and stalks linking the heads to a basal domain, which contains a varying number of accessory intermediate chains. The complex is involved in intracellular transport and motility. The protein described in this record is a light chain and exists as part of this complex but also physically interacts with and inhibits the activity of neuronal nitric oxide synthase. Binding of this protein destabilizes the neuronal nitric oxide synthase dimer, a conformation necessary for activity, and it may regulate numerous biologic processes through its effects on nitric oxide synthase activity. Alternate transcriptional splice variants have been characterized. [provided by RefSeq]. |
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Western blot - DLC8 antibody [EP1660Y]; Anti-DYNLL1 antibody [EP1660Y] at 1/10000 dilution + HeLa cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 10 kDa.Observed band size : 10 kDa.
Immunohistochemistry (Paraffin-embedded sections) - DLC8 antibody [EP1660Y]; Immunohistochemical staining of paraffin embedded human liver using TA303752 (1/100).
Immunocytochemistry/ Immunofluorescence - DLC8 antibody [EP1660Y]; TA303752 staining DLC8 in mouse kidney cells cells by ICC/IF (immunocytochemistry/immunofluorescence. Cells were fixed with methanol, permeabilized with 0.1% Triton and blocked with 1% milk for 1 hour at room temperature. The sample was incubated with primary antibody (1/400; 1% milk in PBS) for 16 hours at 4Â°C. An Alexa Fluor?488-conjugated Goat polyclonal to rabbit IgG (1/1000) was used as secondary antibody.
Immunoprecipitation - Anti-DYNLL1 antibody [EP1660Y]; TA303752 used in IP.SKAP and Astrin form a complex. (A, left) Silver-stained gels showing a one-step IP of GFPLAP-Astrin, GFPLAP-SKAP, or GFPLAP-LC8. (A, right) Data from the mass spectrometric analysis of the purifications indicating the percent sequence coverage from each IP. (B) Silver-stained gel showing the purification of FLAG-SKAP from chicken DT40 cells relative to controls. The indicated proteins were identified by excising them from a gel and analyzing them by mass spectrometry.