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Anti-HIST3H3 Antibody Y49
Also for HIST3H3 (NM_003493)
|A synthetic (Dimethyl-K)-peptide corresponding to residues surrounding Lys9 of Histone H3 was used as immunogen. The antibody only detects Histone H3 dimethylated on Lysine 9. Predicted to cross-react with most species, based on sequence homology.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||ICC/IF: 1:500; ICC: 1:100; WB: 1:1000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt,IHC or IP.
|Homo sapiens histone cluster 3, H3 (HIST3H3)|
|H3.4; H3/g; H3FT; H3t|
|Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. |
EGFR1 Signaling Pathway
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Western blot - Histone H3 (di methyl K9) antibody [Y49]; All lanes : Anti-Histone H3 (di methyl K9) [Y49] antibody at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : recombinant Histone H3.Predicted band size : 15 kDa.Observed band size : 17 kDa .
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K9) antibody [Y49]; ab32521 (1/500) staining Histone H3 di-methyl K9 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details, please refer to Abreview.