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Home Antibody All anti-HIST3H3 antibodies

Anti-HIST3H3 Antibody Y49

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303748
  • Rabbit monoclonal antibody against Dimethyl-Histone H3 (K9)(Y49)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC418646) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IF(1)
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Also for HIST3H3 (NM_003493)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic (Dimethyl-K)-peptide corresponding to residues surrounding Lys9 of Histone H3 was used as immunogen. The antibody only detects Histone H3 dimethylated on Lysine 9. Predicted to cross-react with most species, based on sequence homology.
Clone NameY49 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF Suggested DilutionsICC/IF: 1:500; ICC: 1:100; WB: 1:1000
Predicted MW Explanation 15 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt,IHC or IP.

Reference Data

Target NameHomo sapiens histone cluster 3, H3 (HIST3H3)
Alternative NameH3.4; H3/g; H3FT; H3t
Database LinkNP_003484
Entrez Gene 8290 Human
Entrez Gene 691496 Rat
FunctionHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Related Pathway Systemic lupus erythematosus

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Histone H3 (di methyl K9) antibody [Y49]; All lanes : Anti-Histone H3 (di methyl K9) [Y49] antibody at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : recombinant Histone H3.Predicted band size : 15 kDa.Observed band size : 17 kDa .
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K9) antibody [Y49]; TA303748 (1/500) staining Histone H3 di-methyl K9 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red).

 

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