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Anti-HIST3H3 PHOSPHO Antibody E191
Also for HIST3H3 (NM_003493)
|A synthetic phospho-peptide corresponding to residues surrounding Ser28 of human Histone H3 was used as immunogen. The antibody only detects Histone H3 phosphorylated on Serine 28.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:2000; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:250; FC: 1:100; IHC-FoFr: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified (Protein A or G Sepharose)
|Homo sapiens histone cluster 3, H3 (HIST3H3)|
|H3.4; H3/g; H3FT; H3t|
|Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. |
EGFR1 Signaling Pathway
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Western blot - Histone H3 (phospho S28) antibody [E191]; All lanes : Anti-Histone H3 (phospho S28) antibody [E191] at 1/2000 dilution.Lane 1 : NIH 3T3 cell lysate -untreated.Lane 2 : NIH 3T3 cell lysate -treated with FBS + CalA.Predicted band size : 17 kDa.Observed band size : 17 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Histone H3 (phospho S28) antibody [E191]; Immunohistochemical analysis of Histone H3 (phospho S28) expression in paraffin embedded lymphoma tissue sections, using 1/100 ab32388. left: untreated sample; right: phosphatase treated sample (negative control).
Flow Cytometry - Anti-Histone H3 (phospho S28) antibody [E191]; Overlay histogram showing HeLa cells stained with ab32388 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.