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Anti-HIST3H3 PHOSPHO Antibody E173
Also for HIST3H3 (NM_003493)
|A synthetic phospho-peptide corresponding to residues surrounding Ser10 of Human Histone H3 was used as immunogen. The antibody only detects Histone H3 phosphorylated on Serine 10. Predicted to cross-react with most species, based on sequence homology.|
|Mouse, Rat, Human, Fruit fly (Drosophila melanogaster)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||ICC/IF: 1:1000; WB: 1:2500; IHC-P: Use at an assay dependent dilution; ICC: 1:250; IP: 1:40
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for Flow Cyt.
|Homo sapiens histone cluster 3, H3 (HIST3H3)|
|H3.4; H3/g; H3FT; H3t|
Entrez Gene 8290 Human
Entrez Gene 691496 Rat
|Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. |
| Systemic lupus erythematosus|
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Western blot - Histone H3 (phospho S10) antibody [E173]; All lanes : Anti-Histone H3 (phospho S10) antibody [E173] at 1/2500 dilution.Lane 1 : Hela cell lysate, untreated.Lane 2 : Hela cell lysate, treated with Alkaline Phosphatase.Predicted band size : 15 kDa.Observed band size : 17 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Anti-Histone H3 (phospho S10) antibody [E173]; Ab32107, at a 1/50 dilution, staining Histone H3 in paraffin embedded lymphoma tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Histone H3 (phospho S10) antibody [E173]; TA303743 (1/1000) staining Histone H3 (phospho S10) in paraformaldehyde-fixed, DAPI counterstained HeLa cells. Secondary antibody: Goat anti-Rabbit IgG conjugated to Cy3(1/200).