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Home Antibody All anti-HIST3H3 antibodies

Anti-HIST3H3 PHOSPHO Antibody E173


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA303743 Rabbit monoclonal antibody against Phospho-Histone H3 (pS10)(E173-25) (phospho-specific) 100ul $325 In Stock
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Also for HIST3H3 (NM_003493)
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OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Ser10 of Human Histone H3 was used as immunogen. The antibody only detects Histone H3 phosphorylated on Serine 10. Predicted to cross-react with most species, based on sequence homology.
Clone NameE173 IsotypeIgG
Species ReactivityMouse, Rat, Human, Fruit fly (Drosophila melanogaster) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsICC/IF: 1:1000; WB: 1:2500; IHC-P: Use at an assay dependent dilution; ICC: 1:250; IP: 1:40
Predicted MW Explanation 15 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens histone cluster 3, H3 (HIST3H3)
Alternative NameH3.4; H3/g; H3FT; H3t
Database LinkNP_003484
Entrez Gene 8290 Human
Entrez Gene 691496 Rat
FunctionHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Related Pathway Systemic lupus erythematosus

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Histone H3 (phospho S10) antibody [E173]; All lanes : Anti-Histone H3 (phospho S10) antibody [E173] at 1/2500 dilution.Lane 1 : Hela cell lysate, untreated.Lane 2 : Hela cell lysate, treated with Alkaline Phosphatase.Predicted band size : 15 kDa.Observed band size : 17 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Anti-Histone H3 (phospho S10) antibody [E173]; Ab32107, at a 1/50 dilution, staining Histone H3 in paraffin embedded lymphoma tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Histone H3 (phospho S10) antibody [E173]; TA303743 (1/1000) staining Histone H3 (phospho S10) in paraformaldehyde-fixed, DAPI counterstained HeLa cells. Secondary antibody: Goat anti-Rabbit IgG conjugated to Cy3(1/200).


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