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Anti-ZAP70 PHOSPHO Antibody EPR1073
Also for ZAP70 (NM_001079)
|A phospho-specific peptide corresponding to residues surrounding Tyrosine 292 of human ZAP70 was used as an immunogen. This antibody only detects ZAP70 phosphorylated on Tyrosine 292.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:5,000 -10,000; ICC: 1:250 -500; FC: 1:20
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens zeta-chain (TCR) associated protein kinase 70kDa (ZAP70), transcript variant 1|
|SRK; STCD; STD; TZK; ZAP-70|
|This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq]. |
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Western blot - ZAP70 (phospho Y292) antibody [EPR1073]; All lanes : Anti-ZAP70 (phospho Y292) antibody [EPR1073] at 1/10000 dilution.Lane 1 : Jurkat cell lysate (untreated).Lane 2 : Jurkat cell lysate, treated with pervanadate.Lysates/proteins at 10 µg per lane.Secondary.goat anit-rabbit HRP labeled IgG at 1/2000 dilution.Predicted band size : 70 kDa.Observed band size : 70 kDa.
Flow Cytometry - ZAP70 (phospho Y292) antibody [EPR1073]; Flow cytometric analysis of permeabilized Jurkat cells, untreated (green), pervanadate treated (red) stained with ab76501, at 1/20 dilution (blue) and pervanadate-treated Jurkat cells using the same antibody preincubated with phospho- ZAP70 (Tyr 292) peptide (blue) or non-phospho- ZAP70 (Tyr 292)).