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Anti-WAS Antibody EP2541Y
|A synthetic peptide corresponding to residues near the C-terminus of human WASP was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:10000 - 1:50000; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:10 - 1:100
|Does not react with Mouse, Rat. Is unsuitable for IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens Wiskott-Aldrich syndrome (WAS)|
|IMD2; SCNX; THC; THC1; WASP|
|The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known. [provided by RefSeq]. |
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Western blot - WASP antibody [EP2541Y]; All lanes : Anti-WASP antibody [EP2541Y] at 1/50000 dilution.Lane 1 : TF-1 cell lysate.Lane 2 : U937 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 53 kDa.Observed band size : 60 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - WASP antibody [EP2541Y]; ab75830 at 1/100 dilution staining WASP in paraffin-embedded human spleen tissue.
Flow Cytometry - Anti-WASP antibody [EP2541Y]; Flow cytometry analysis of permeabilized U937 cells, staining WASP (red) with at 1/10 dilution. Rabbit IgG was used as negative control (green).