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Anti-VIM PHOSPHO Antibody EP1070Y
|A synthetic acetylated peptide corresponding to residues surrounding Ser72 of Vimentin was used as an immunogen. The antibody only detects Vimentin phosphorylated at Serine 72.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1e+006; IP: 1:40
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt,IHC-Fr or IHC-P.
|Homo sapiens vimentin (VIM)|
|Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]. |
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Western blot - Vimentin (phospho S72) antibody [EP1070Y]; All lanes : Anti-Vimentin (phospho S72) antibody [EP1070Y] at 1/1000000 dilution.Lane 1 : HeLa cell lysate untreated.Lane 2 : HeLa cell lysate treated with Calyculin A.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 54 kDa.Observed band size : 54 kDa.