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Anti-UBTF Antibody EP2741Y
Also for UBTF (NM_001076683)
|A synthetic peptide corresponding to residues near the N-terminus of human UBF was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:500; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:30 - 1:50
|Does not react with Rat. Is unsuitable for IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens upstream binding transcription factor, RNA polymerase I (UBTF), transcript variant 2|
|NOR-90; UBF; UBF-1; UBF1|
|Upstream binding factor (UBF) is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1 (a complex of TBP (MIM 600075) and multiple TBP-associated factors or 'TAFs'). Two UBF polypeptides, of 94 and 97 kD, exist in the human (Bell et al., 1988 [PubMed 3413483]). UBF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxes (Jantzen et al., 1990 [PubMed 2330041]).[supplied by OMIM]. |
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Western blot - UBF1 antibody [EP2741Y]; All lanes : Anti-UBF1 antibody [EP2741Y] at 1/500 dilution.Lane 1 : (A) HeLa cell lysate.Lane 2 : (B) A431 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 89 kDa.Observed band size : 89 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UBF1 antibody [EP2741Y]; Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab75781 at 1/100-1/250 dilution.
Flow Cytometry - Anti-UBF1 antibody [EP2741Y]; Overlay histogram showing A431 cells stained with ab75781 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.